丹参对内皮素-1介导的肝星状细胞Ca2+的影响机制
http://www.100md.com
姚冬梅, 房澍名, 杨川杰, 修贺明, 苏素文,
丹参;内皮素-1;钙;肝星状细胞,丹参对内皮素-1介导的肝星状细胞Ca2+的影响机制,姚冬梅,房澍名,杨川杰,姚希贤,修贺明,苏素文,姚冬梅,通讯作者,Effectsofradixsalviaemil
 |
| 第1页 |
参见附件(893KB，5页)。
姚冬梅, 房澍名, 杨川杰, 姚希贤, 河北医科大学第二医院 河北省石家庄市 050000
修贺明, 中国人民解放军白求恩国际和平医院细胞室 河北省石家庄市 050082
苏素文, 河北医科大学基础医学院药理教研室 河北省石家庄市 050017
姚冬梅, 2003年河北医科大学博士, 主治医师, 主要从事慢性肝病研究.
通讯作者: 姚冬梅, 050000, 河北省石家庄市和平西路215号, 河北医科大学第二医院消化内科. ydm8080@126.com
电话: 0311-87222951
收稿日期: 2006-02-16 接受日期: 2006-03-03
Effects of radix salviae miltiorrhizae on endothelin-1-induced calcium changes in hepatic stellate cells
Dong-Mei Yao, Shu-Ming Fang, Chuan-Jie Yang, He-Ming Xiu, Su-Wen Su, Xi-Xian Yao
Dong-Mei Yao, Shu-Ming Fang, Chuan-Jie Yang, Xi-Xian Yao,Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
He-Ming Xiu, Department of Cytology,Chinese PLA Bethune International Peace Hospital, Shijiazhuang 050082, Hebei Province, China
Su-Wen Su,Department of Pharmacology, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
Correspondence to:Dr. Dong-Mei Yao, Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. ydm8080@126.com
Received:2006-02-16 Accepted:2006-03-03
Abstract
AIM: To investigate the effect of radix salviae miltiorrhizae (RSM) on the changes of [Ca2+]i induced by endothelin-1 (ET-1) in hepatic stellate cells (HSCs).
METHODS: After preparation of RSM cream, the effects of RSM on ET-1-induced changes of HSCs [Ca2+]i were observed by laser scanning confocal microscopy.
RESULTS: In normal buffer (including Ca2+, buffer A), the fluorescence intensity was enhanced accordingly with the increase of ET-1 concentration. The cumulative-response curve showed EC50 was 1.1×10-9 mol/L. After incubation of HSCs with ET-1 in buffer A and buffer B (absence of extracellular calcium, EGTA), the duration of calcium peak had significant difference (165 ......
您现在查看是摘要介绍页，详见PDF附件(893KB，5页)。