Effect of Dragon¬ðs blood extracts on proliferation of human fibroblast cells

    LI Dan, GUO Shuª²Zhong, WANG Shengª²Chun, ZHANG Linª²Xi, HU Yongª²Wu

    Department of Plastic Surgery, Department of Pharmaceutics, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of Dragon¬ðs blood on the proliferation of fibroblast cells in vitro and to explore the mechanism by which Dragon¬ðs blood promotes wound healing. METHODS: Human fibroblast cells were cultured in vitro in media containing gradient concentrations of 3 kinds of Dragon¬ðs blood extracts (extracted by chloroform, acetidin and alcohol, respectively), which was followed by cell proliferation determination assessed with MTT assay at different time intervals. The growth curve at the most suitable concentration was drawn. The flow cytometry (FCM) was used to determine the changes of cell cycle at this concentration. RESULTS: The 0.5-2.0 g/L dilution of Dragon¬ðs blood extract by acetidin enhanced the proliferation of fibroblast in a doseª²dependent manner. The 2.0 g/L was the optimal concentration of Dragon¬ðs blood extract, and it could increase the cell ratio in Sª²phase of cell cycle by 18.3% of that in control group (P<0.01). CONCLUSION: Dragon¬ðs blood extract by acetidin could promote the proliferation of cultured human fibroblast cells in vitro, and it might be beneficial to granulation tissue formation during wound healing. ......