信号放大时间分辨荧光分析技术的研究进展及应用
信号放大；时间分辨荧光分析；镧系元素螯合物,,信号放大；时间分辨荧光分析；镧系元素螯合物,1信号放大时间分辨荧光分析技术的原理及特点,2生物素—链
【摘要】 信号放大时间分辨荧光分析技术是一种新型的超微量的标记分析技术。它是以生物信号放大为基础，以镧系元素螯合物为标记物，以时间分辨和波长分辨为测量方法，充分利用了酶标记分析和PCR扩增等分析技术优点的技术。它的独特优点是非同位素和超灵敏度。本综述介绍了TRFA的特点以及酶放大、二次酶放大、铕纳米颗粒、滚动循环放大、免疫PCR和荧光淬灭分析等信号放大时间分辨荧光分析技术的研究进展及应用。信号放大时间分辨荧光分析技术在生物分析中极其广泛的应用，显示出具有很好的发展前景。【关键词】 信号放大；时间分辨荧光分析；镧系元素螯合物
Studies and applications of time-resolved fluorescence assay based on signal amplification
SONG Na-ling, ZHAO Qi-ren.
The Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China
【Abstract】 Time-resolved fluorescence assies(TRFA) based on signal amplification are new ultrasensitive labelling assay technologies.The basis is biosignal amplification,the labels are lanthanide chelates,and the detection methods are the time-resolved and wavelength-resolved fluorescence assay in the technologies.In addition,enzyme-labelled assay and PCR amplification et al are used in the technologies.The several main signal amplification methods and amplifications, for example, enzyme-amplification, two-round enzyme-amplification,europium nanopartical,rolling circle amplification,immuno-PCR and fluorescence quenching assay et al, were introduced in the summary. The unique advantages of nonisotope and ultrasensitivity and extensive applications in bioassay shown good prospects of TRFA based on signal amplification. ......
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