作者：章军建 阮旭中 汪银洲 田有勇

    单位：430030 武汉 同济医科大学附属同济医院神经内科

    关键词：脑缺血；神经生长因子；细胞凋亡；海马

    摘 要 目的 研究神经生长因子(NGF)在缺血性脑损伤中对神经细胞凋亡的影响。方法 采用大鼠4血管闭塞脑缺血模型，TUNEL方法原位标记DNA片段，观察脑室内注射NGF后海马CA₁区神经细胞凋亡的变化。结果 使用NGF后3d海马CA₁区TUNEL阳性神经细胞数为神经细胞总数的13.9%±11.6%，与缺血对照组(21.3%±13.7%)比较显著减少(P<0.01)。结论 外源性NGF对脑缺血所致的神经细胞凋亡可能具有一定的保护作用。

    Effect of nerve growth factor on neuronal apoptosis in hippocapal CA₁ sector after global ischemia in rats

    Zhang Junjian,Ruan Xuzhong，Wang Yingzhou,et al.

    (Department of Neurology,Tongji Hospital,Wuhan,430030)

    Abstract Objective To investigate the protective action of nerve growth factor (NGF) on neuronal apoptosis by ischemic insults.Methods Global ischemia in Wistar rats was induced using four-vessel occlusion,and NFG group intraventricular adminstration of NGF after ischemia,control group received the same volue of solvent.At the 3 days after ischmia,terminal deoxynucleotidyl transferase mediated dUTP-biotion nick endlabeling (TUNEL) hippocampal CA₁ sector.Result The results showed that the number of TUNEL positive cells to the number of whole cells in the CA₁ subified was 21.3±13.7% in NGF group,and 13.9%±11.6% in control group,significant difference between the ratios in two group (P<0.01).Conclusion It was suggested that intraventricular NGF has a protective effect on neuronal appoptosis by ischemic insults. ......