树突状细胞致敏的肿瘤疫苗对胰腺癌细胞的杀伤效应
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陈 鑫, 杨 帆, 杨兴无, 张 帆
树突状细胞;细胞毒性T淋巴细胞;胰腺癌细胞;细胞毒效应陈鑫,杨帆,杨兴无,张帆.树突状细胞致敏的肿瘤疫苗对胰腺癌细胞的杀伤效应. 世界华人消化杂志2006;14(28)2815-2818&
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陈鑫, 杨兴无, 张帆, 大连医科大学附属第二医院普外科 辽宁省大连市 116027
杨帆, 大连医科大学生物化学与分子生物学教研室 辽宁省大连市 116027
通讯作者: 陈鑫, 116027, 辽宁省大连市, 大连医科大学附属第二医院普外科. xinchen26@yahoo.com.cn
电话: 0411-84671291-3063
收稿日期: 2006-07-08 接受日期: 2006-08-10
Killing effect of tumor vaccine pulsed with dendritic cells on pancreatic carcinoma cells
Xin Chen, Fan Yang, Xing-Wu Yang, Fan Zhang
Xin Chen, Xing-Wu Yang, Fan Zhang, Department of General Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China
Fan Yang, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, Liaoning Province, China
Correspondence to: Xin Chen, Department of General Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province,China. xinchen26@yahoo.com.cn
Received: 2006-07-08Accepted: 2006-08-10
Abstract
AIM: To investigate the anti-tumor effect of cytotoxic T lymphocytes (CTLs) in vitro induced by human dendritic cells (DCs) sensitized with pancreatic carcinoma cell lysate on the primarily cultured autogenous cells.
METHODS: Pancreatic carcinoma cells from 6 patients were lysed as tumor antigens. T cells and DCs were separated from peripheral blood ......
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