http://www.100md.com 《分析化学》 2006年第10期
分子印迹聚合物,大体积直接进样,双酚A,高效液相色谱法,,分子印迹聚合物，大体积直接进样，双酚A，高效液相色谱法,1引言,2实验部分,3结果与讨论,Ref摘 要 合成单分散双酚A(BPA)分子印迹微球并以其为高效液相色谱柱填充材料，建立了一种大体积直接进样HPLC法测定环境水中超痕量BPA的方法。方法的检出限(LOD)和测定限(LOQ)分别为0.03 nmol/L和0.1 nmol/L;在0.1～100 nmol/L范围内具有良好的线性(r2=0.9983)。本方法用于表层湖水中BPA的检测，实际样品的加标回收率在99% ～101.4%间;相对标准偏差(RSD %)低于8.1%(n＝5)。
Determination of Ultratrace Bisphenol A in Water Samples by Chromatography with Large Volume Direct Injection
Jiang Ming, Lin Yi，Zhang Jianghua，Mei Surong, Lü Bin
1Key Laboratory of Environment & Health of Ministy of Education, Institute of Environmental Medicine,School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030
2School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030
Abstract A method was developed for the determination of ultratrace bisphenol A (BPA) in water sample by chromatography with large volume (40 mL) direct injection without pretreatment. The enrichment and separation of BPA in water were achieved on a BPA imprinted polymer microspheres packed in a high performance liquid chromatography column. The calibration graph was linear with R2>0.998 in the ranges of 0.1-100 nmol/L BPA standard solution. The limit of detection and limit of quantitation were 0.03 nmol/L and 01 nmol/L, respectively. For spiked lake water analysis, the RSD (N=5) was less than 8.1% and the recoveries were 96%-101.4%. ......
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