缺氧诱导因子-1α在胰腺癌中的作用
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陈干涛, 白少华, 高志强
缺氧诱导因子-1a;血管内皮生长因子;葡萄糖转运体1;多耐药相关基因1陈干涛,白少华,高志强.缺氧诱导因子-1a在胰腺癌中的作用. 世界华人消化杂志2006;14(30)2965-2970
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陈干涛, 第一人民医院消化内科 湖北省仙桃市 443000
白少华, 第一人民医院普通外科 湖北省仙桃市 443000
高志强, 华中科技大学同济医学院附属同济医院普通外科 湖北省武汉市 430032
通讯作者: 陈干涛, 443000, 湖北省仙桃市, 湖北省仙桃市第一人民医院消化内科. doctor_nancy518@yahoo.com
电话: 0728-3242554
收稿日期: 2006-04-17 接受日期: 2006-05-11
Role of hypoxia-inducible factor-1α in pancreatic cancer
Gan-Tao Chen, Shao-Hua Bai, Zhi-Qiang Gao
Gan-Tao Chen, Department of Digestive Diseases, the First People’s Hospital, Xiantao 443000, Hubei Province, China
Shao-Hua Bai, Department of General Surgery, the First People’s Hospital, Xiantao 443000, Hubei Province, China
Zhi-Qiang Gao, Department of General Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, Hubei Province, China
Supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region, China, No. No. 0447054
Correspondence to: Gan-Tao Chen, Department of Digestive Diseases, the First People’s Hospital, Xiantao 443000, Hubei Province, China. doctor_nancy518@yahoo.com
Received: 2006-04-17 Accepted: 2006-05-11
Abstract
AIM:To study the level changes of hypoxia-inducible factor-1a (HIF-1a), vascular endothelial growth factor (VEGF), glucose transporter 1 (GLUT1) and multidrug resistance gene (MDR1) in pancreatic carcinoma cells SW1990 exposed to hypoxia, and to explore the possible molecular mechanism.
METHODS: CoCl2 was used to induce chemical hypoxia, and SW1990 cells were divided into normal oxygen group and hypoxia group. The expression of HIF-1a, VEGF, GLUT1 and MDR1 at the mRNA and protein levels were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blot, respectively, in the above groups 24 h after treatment with hypoxia and transfected with plasmid HIF-1a/PCDNA3.
RESULTS: Chemical hypoxia induced significant expression of HIF-1a protein (P = 0 ......
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