人乳腺癌ZR751细胞；_PTEN基因；细胞周期

转染抑癌基因PTEN对人乳腺癌ZR751细胞的细胞周期和增殖能力的影响

http://www.100md.com 《广东药学院学报》 2006年第2期

人乳腺癌ZR751细胞；,PTEN基因；细胞周期,,人乳腺癌ZR751细胞；,PTEN基因；细胞周期,1材料与方法

     【摘要】目的：观察PTEN(phosphatase and tensin homology deleted on chromosome ten，第10号染色体上磷酸酶和张力蛋白同源缺失的基因)对人乳腺癌细胞ZR751细胞增殖和细胞周期的影响。方法：利用脂质体介导法将携带有野生型和突变型PTEN cDNA的真核表达载体pBPwtPTEN和pBPG129RPTEN导入人乳腺癌ZR751细胞(质粒转染成功后实验分为CWTPTEN组、CG129RPTEN组和未转染质粒组即对照组)后，以RTPCR、Western blot 分析目的基因的表达，并采用MTT法和流式细胞术检测细胞增殖和细胞周期。结果：CWTPTEN组、CG129RPTEN组细胞PTEN mRNA 及PTEN蛋白出现明显的高表达。CWTPTEN组细胞生长的抑制率可高达42.7%，与对照组比较，差异有显著性(P<0.05)。但CG129RPTEN组细胞生长的抑制率与对照组比较，差异无显著性(P>0.05)。流式细胞术显示CWTPTEN组细胞周期从G1期到S期已发生抑制。结论：野生型PTEN可依赖其磷酸酶活性抑制肿瘤细胞的增殖，并最终诱导细胞凋亡。

    【关键词】人乳腺癌ZR751细胞； PTEN基因；细胞周期

    Effect of the transfected PTEN gene on cell cycle and proliferation of

    human breast cancer cell line

    LI Xiangyong , LIN Guangping , ZHOU Keyuan

    (Department of Biochemistry And Molecule Biology，Guangdong Medical College， Zhanjiang 524023，China)

    【Abstract】 Objective: To investigate the effect of the PTEN (phosphatase and tensin homologue deleted on chromosome ten) gene on cell cycle and proliferation in human breast cancer cell line ZR751. Methods: The eukaryotic expression vector pBPwtPTEN and pBPG129RPTEN containing PTEN cDNA was transfected into ZR751 cells by lipofectamine. Three groups were set: CWTPTEN, CG129RPTEN and cells without transfection as the control group. Reverse transcriptionpolymerase chain reaction and Westernblot methods were used to detect PTEN expression in the transfected ZR751 cells. The cell cycle, cell proliferation and cell apoptosis of the ZR751 cells was determined by the MTT and flow cytometry. Results: High expression of PTEN mRNA and protein was seen in the transfected ZR751 cells. The proliferation of CWTPTEN cells were inhibited by 42.7% (vs the control group, P< 0.05), while CG129RPTEN cells showed no significant change in proliferation (vs the control group, P> 0.05). The cell cycle from G1 to S of CWTPTEN cells was inhibited. Conclusion: Wide type PTEN gene might suppress the growth and induces the apoptosis in human breast cancer cell line ZR751 cells through its phosphatase activity. ......

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