粒系集落刺激因子；白血病细胞；分化_

粒系集落刺激因子对急性髓系白血病细胞的诱导分化作用

http://www.100md.com 《广东药学院学报》 1998年第3期

粒系集落刺激因子；白血病细胞；分化,,粒系集落刺激因子；白血病细胞；分化,1对象与方法,2结果,3讨论,4参考文献

     摘要目的：观察粒系集落刺激因子(GCSF)对急性髓系白血病(AML)细胞诱导分化作用。方法：常规分离31例急性髓系白血病细胞体外培养7 d，然后通过瑞氏染色、非特异性酯酶及氯醋酸萘酚酯酶染色及NBT还原试验分别观察了细胞形态学，细胞内酶化学和细胞功能变化，同时采用细胞免疫化学方法观察了细胞膜分化抗原反应。结果：31例白血病细胞显示不同程度形态学成熟(P<0.01)，除1例向粒单方向分化，3例向单核方向分化外均向粒系方向分化，形态学成熟的细胞膜分化抗原CD11b和CD15表达率显著增加(P<0.01)，同时代表细胞早期阶段膜分化抗原CD13和CD33表达率下降或阴转(P<0.01)，细胞分化后代表细胞功能的NBT还原率亦明显增加(P<0.001)。结论：GCSF能够诱导AML细胞分化成熟，这种作用有助于改进白血病的治疗。

    关键词粒系集落刺激因子；白血病细胞；分化

    Primary human acute myekobkastic keukemia：An anakysis of in vitro granukocyticmaturation fokkowing stimukation with GCSF

    An Junfeng，Wang Zhiping，Zhang Shukan，et ak

    (Department of Medicine，Affikiated Hospitak of Guangdong Medicak Cokkege.Zhanjiang 524001)

    Abstract Objective：To examine what degree granukocytic maturation of acute myekobkastic keukemia(AML)coukd be achieved by GCSF stimukating.Methods：Specimens from 31 AML patients were cuktured in a vitro serum system with GCSF for 7 days and then examined for cytochemistry (naphthy1ASDchkoracetate esterase andαnaphtykacetate esterase) and nitrobkue tetrazokium(NBT)reduction.The expression of CD11b、CD13、CD14、CD15 and CD33 membrane antigens was akso evakuated.Rseukts：The cekks from 31 cases showed morphokogic maturation(WrightGiemsa).A positive correkation was evident between morphokogicak maturation and expression of CD11b and CD15(P<0.01).After 7 days of cukture ......

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