Detection of HPV16 E7 gene nt647 point mutation in cervical cancerous tissue using TDIª²FP technique

    FAN Jiangª²Bo1, QU Qun, WU Jing, ZHANG Ju, GAO Yanª²E, ZHANG Geª²Lin

    1Department of Obstetrics and Gynecology, Second Affiliated Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 710004, China

    2Institute of Gene Diagnostics, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM£º To detect HPV16 E7 nt647 point mutation in cervical cancerous tissue using TDIª²FP technique in order to provide valuable clinical data for warning, detection, diagnosis, gene therapy, prevention and prognosis of cervical cancer and precancerous lesion with HPV infections. METHODS£ºTDIª²FP technique is a compounded reaction including PCR, probe hybridization and base incorporation with high specificity and sensitivity. We detected the point mutation of HPV16 E7 nt647 in the detected HPV 16 positive cervical cancerous tissue by the TDIª²FP method, which can explain the correlation between the point mutation and cervical cancer. RESULTS£º The HPV16 E7 nt647 point mutation rate was 32.35% in the study. The point mutation rate of HPV16 E7 nt647 was significantly different from that of the control group (¦Ö2=12.437 ......