comClonality of squamous intraepithelial lesion of cervix

    ZHU Shaoª²Jun, LI Yanª²Hong, ZHANG Wei, ZHAO Hongª²Xi, GONG Li, LAN Miao, LI Aiª²Ning

    1Department of Pathology

    2Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To demonstrate the clonality status of squamous intraepithelial lesion (SIL) of cervix using an assay based on inactivation mosaicism of the lengthª²polymorphic X chromosomes at the androgen receptor (AR) locus. METHODS: Proliª²ferative epithelial component of the SIL was collected by using microdissection from the routine sections stained with hematoxylin and eosin. Stromal component was also isolated from the same samples, and used for parallel controls. Genomic DNA was extracted, pretreated with Hha I, and amplified via nested PCR. The products were resolved on denaturing polyacrylamide gels and visualized through silver staining. RESULTS: A total of 73 cases were examined, including 26 samples of CIN¢ñ, 23 samples of CIN ¢ò and 24 samples of CIN ¢ó. All the samples was successfully amplified and 65 of them (24 samples of CIN¢ñ, 20 samples of CIN¢ò and 21 samples of CIN ¢ó) carried the length polymorphism at the AR locus. Monoclonal composition was demonstrated in 2 of 24 samples of CIN¢ñ, 13 of 20 samples of CIN¢ò and 19 of 19 samples of CIN¢ó. Mitotic indices of the monoclonal lesions were higher than those of the polyclonal lesions, but no difference was found between their apoptotic body counts. CONCLUSION: In cervix, most of CIN ¢ñ and some of CIN¢ò are polyclonal, thereby being responsive proliferative lesions. Most of the CIN¢ò and all of CIN¢ó show monoclonal cell expansion, and they may be already neoplastic. ......