化学合成经修饰抗TIMP-2小干扰RNA对CCl4诱导肝纤维化动物模型的影响
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胡迎宾, 李定国, 李光明, 孙 超, 徐芹芳
金属蛋白酶组织抑制剂-2;小干扰RNA;肝纤维化;基因治疗胡迎宾,李定国,李光明,孙超,徐芹芳.化学合成经修饰抗TIMP-2小干扰RNA对CCl4诱导肝纤维化动物模型的影响. 世界华人消化
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胡迎宾, 李定国, 李光明, 孙超, 徐芹芳, 上海交通大学医学院附属新华医院消化内科 上海市 200092
胡迎宾, 2004年华中科技大学同济医学院硕士, 住院医师, 主要从事慢性肝病的研究.上海市重点学科建设资助项目, No. Y0205
通讯作者: 李定国, 200092, 上海市, 上海交通大学医学院附属新华医院消化内科. dingguo_li@263.net
电话: 021-65790000-3361
收稿日期: 2006-07-23 接受日期: 2006-09-28
Effects of chemically modified small interfering RNA targeting tissue inhibitor of metalloproteinase-2 on carbon tetrachloride-induced liver fibrosis in rats
Ying-Bin Hu, Ding-Guo Li, Guang-Ming Li, Chao Sun, Qin-Fang Xu
Ying-Bin Hu, Ding-Guo Li, Guang-Ming Li, Chao Sun, Qin-Fang Xu, Department of Digestive Diseases, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China
Correspondence to: Professor Ding-Guo Li, Department of Digestive Diseases, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China. dingguo_li@263.net
Received: 2006-07-23 Accepted: 2006-09-28
Abstract
AIM: To probe the effects of chemically modified small interfering RNA (siRNA) targeting tissue inhibitor of metalloproteinase-2 (TIMP-2) on liver fibrosis as well as its possible mechanism in rats.
METHODS: A total of 42 male rats were randomly and averagely divided into 7 groups: a normal group, a negative control group, a sham-operated group, a model group and three siRNA-treated groups (treated with 0.05, 0.1, and 0.2 mg/kg siRNA via portal vain, respectively). Carbon tetrachloride (CCl4) was used to induce liver cirrhosis. All the rats were sacrificed at 8 wk to collect liver tissue specimens, and the portal vein pressure (PVP) was measured. The pathological changes of liver tissues were observed after HE and Van Gieson (VG) staining. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN), procolagen type Ⅲ (PCⅢ), collagen type Ⅳ (CIV) and hydroxyproline (Hyp) were measured ......
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