亚砷酸联合重组腺病毒Ad-IκBaM对肝细胞癌的治疗作用
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刘 丹, 刘冰熔, 胡丽红, 杜雅菊, 裴凤华,
核转录因子kB;肝癌;亚砷酸;基因治疗;重组腺病毒,刘丹,刘冰熔,胡丽红,杜雅菊,裴凤华,吕志武,关景明,刘丹,通讯作者:,Thera
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刘丹, 刘冰熔, 胡丽红, 杜雅菊, 裴凤华, 吕志武, 关景明, 哈尔滨医科大学附属二院消化内科 黑龙江省哈尔滨市 150086
刘丹, 2005年毕业于哈尔滨医科硕士, 住院医师, 主要从事消化道肿瘤的基础及临床研究.
黑龙江省科学技术计划项目, No. GC02C148-01
通讯作者: 刘冰熔, 150086, 黑龙江省哈尔滨市南岗区学府路246号, 哈尔滨医科大学附属二院消化内科.
liubingrong@medmail.com.cn
电话: 0451-86605980 传真: 0451-86605980
收稿日期: 2006-09-19 接受日期: 2006-10-11
Therapeutic effect of arsenious acid combined with recombinant adenovirus Ad-IκBαM on hepatocellur carcinoma
Dan Liu, Bing-Rong Liu, Li-Hong Hu, Ya-Ju Du, Feng-Hua Pei, Zhi-Wu Lv, Jing-Ming Guan
Dan Liu, Bing-Rong Liu, Li-Hong Hu, Ya-Ju Du, Feng-Hua Pei, Zhi-Wu Lv, Jing-Ming Guan,Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Heilongjiang Province, China
Supported bythe Science and Technology Project of Heilongjiang Province, No. GC02C148-01
Correspondence to:Dr. Bing-Rong Liu, Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Street, Harbin 150086, Heilongjiang Province, China. liubingrong@medmail.com.cn
Received:2006-09-19 Accepted:2006-10-11
Abstract
AIM: To observe the inhibitory effect of recombinant adenovirus Ad-IκBaM on the activation of nuclear factor kappa B (NF-κB) as well as the enhancing effect of arsenious acid on the apoptosis of hepatocellular carcinoma cells.
METHODS: Hepatocellular carcinoma cell lines BEL-7402 and SMMC-7721 were treated with different concentrations of arsenious acid, respectively. The recombinant adenoviruses were prepared to transfect BEL-7402 and SMMC-7721 cells received or not received arsenious acid treatment. MTT assay and TUNEL method were used to observe the growth and apoptosis of the cells, respectively. Electrophoretic mobility shift assay (EMSA) and Western blot were performed to detect the activation of NF-κB and its inhibition after Ad-IκBaM transfection, respectively ......
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