髓源性树突状细胞过继转移诱导1型糖尿病小鼠免疫耐受及其机制
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张程亮, 向 明, 邹晓蕾, 彭佳蓓
树突状细胞;1型糖尿病;免疫耐受;调节性T细胞,张程亮,向明,邹晓蕾,彭佳蓓,张程亮,通讯作者:,Immunetolerancean
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张程亮, 向明, 邹晓蕾, 彭佳蓓, 华中科技大学同济医学院药学院药理室 湖北省武汉市 430030
张程亮, 2004年华中科技大学同济医学院硕士, 主要从事免疫药理学研究.
国家自然科学基金资助项目, No. 30200343
通讯作者: 向明, 430030, 湖北省武汉市, 华中科技大学同济医学院药学院药理教研室. xiangming@mails.tjmu.edu.cn
电话: 027-83657813 传真: 027-83657547
收稿日期: 2006-08-23 接受日期: 2006-09-21
Immune tolerance and its mechanism induced by adoptive transfer of bone marrow-derived dendritic cells in mice with insulin-dependent diabetes mellitus
Cheng-Liang Zhang, Ming Xiang, Xiao-Lei Zou, Jia-Bei Peng
Cheng-Liang Zhang, Ming Xiang, Xiao-Lei Zou, Jia-Bei Peng,Department of Pharmacology, College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported byNational Natural Science Foundation of China, No. 30200343
Correspondence to:Ming Xiang, Department of Pharmacology, College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. xiangming@mails.tjmu.edu.cn
Received:2006-08-23 Accepted:2006-09-21
Abstract
AIM: To investigate immune tolerance induced by adoptive transfer of bone marrow-derived dendritic cells (DCs) in mice with type 1 diabetes, and to explore its underlying mechanism.
METHODS: Bone marrow cells from BALB/c mice were used to generate DCs by in vitro co-culturing with cytokines. After identifying the purity of DCs, We injected the cells into BALB/c mice intraperitoneally. The mouse model of type 1 diabetes was established by injecting low dose of streptozotocin (STZ) for 5 consecutive days. The blood glucose was examined once a week. At the end of the 4th week, all the mice were killed and splenic lymphocytes were collected. After in vitro culturing, the proliferation of lymphocytes was detected by MTT assay, and the proportion of CD4+CD25+T cells was analyzed by fluorescence activated cell sorter (FACS) ......
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