钾维普对未成熟心肌细胞缺血-再灌注损伤保护作用
心脏停搏液,心肌保护,缺血-再灌注损伤,维拉帕米,普萘洛尔,,心脏停搏液,心肌保护,缺血-再灌注损伤,维拉帕米,普萘洛尔,1材料和方法,2结果
【摘要】 目的 研究钾维普停搏液对未成熟心肌细胞缺血-再灌注损伤的保护作用。方法 以乳鼠未成熟心肌细胞为材料建立缺血-再灌注损伤模型,从心肌细胞搏动频率、细胞生存率、心肌酶学等方面观察钾维普停搏液对心肌细胞缺血-再灌注损伤的保护作用。结果 与模型组比较,钾维普组肌酸激酶、丙二醛释放量明显降低,心肌细胞搏动频率、细胞生存率及超氧化物歧化酶活性明显升高。结论 钾维普停搏液显著减轻未成熟心肌细胞缺血-再灌注损伤,效果优于高钾液或维拉帕米、普萘洛尔的单独作用。【关键词】 心脏停搏液 心肌保护 缺血-再灌注损伤 维拉帕米 普萘洛尔
The Protective Effect of Kalium-Verapamil-Propranolol Cardioplegic Solution on Cultured Immature Cardiac Myocytes Injured by Ischemia-Reperfusion
Wu Zhijuan,et al.
(Division of Physiology and Pathophsiology,Fujuang Medical University,Fuzhou 35004,China)
【Abstract】 Objective To study the protective effect of kalium-verapamil-propranolol(KVP) cardioplegia on cultured immature rat cardiac myocytes injured by ischemia-reperfusion. Methods The ischemia-reperfusion injury model of cultured neonatal rat cardiac myocytes was developed. The spontaneous beating,surviving rate,the content of Creatinkinase,Superoxide Dismutase,Malondialdehyde in supernatant after ischemia-reperfusion were detected. Results Compared with the measurements taken from ischemia-reperfusion model group,the CK and MDA in supernatant decreased sharply; the spontaneous beating,surviving rate of cardiac myocytes and the activity of SOD increased highly. Conclusions The KVP cardioplegia can obviously lessen the injury of ischemia-reperfusion in immature cardiac myocytes. The KVP cardioplegia is superior to hyperkalium cardioplegia,verapamil and propranolol. ......
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