人食管癌顺铂耐药细胞系Ec9706/cDDP的建立及其生物学特征
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李 敏, 王志举, 李文涛, 董子明
食管癌;耐药细胞系;顺铂;细胞周期;四甲基偶氮唑蓝;流式细胞术李敏,王志举,李文涛,董子明.人食管癌顺铂耐药细胞系Ec9706,cDDP的建立及其生物学特征. 世界华人消化杂志2006;1
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李敏, 郑州大学基础医学院微生物学与免疫学教研室 河南省郑州市 450052
王志举, 郑州大学基础医学院生理学教研室 河南省郑州市 450052
李文涛, 郑州大学第一附属医院普外科 河南省郑州市 450052
董子明, 郑州大学基础医学院病理生理学教研室 河南省郑州市 450052
李敏, 郑州大学基础医学院博士, 讲师, 主要从事病理生理学研究.
通讯作者: 董子明, 450052, 河南省郑州市, 郑州大学基础医学院病理生理学教研室. dongzm@zzu.edu.cn
收稿日期: 2006-08-28 接受日期: 2006-09-20
Establishment of a cisplatin-induced human esophageal carcinoma drug-resistant cell line and its biological characteristics
Min Li, Zhi-Ju Wang, Wen-Tao Li, Zi-Ming Dong
Min Li, Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province, China
Zhi-Ju Wang, Department of Physiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province, China
Wen-Tao Li, Department of General Surgery, the First Affiliated Hospital of Zhenzhou University, Zhengzhou 450052, Henan Province, China
Zi-Ming Dong, Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province, China
Correspondence to: Zi-Ming Dong, Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province,China. dongzm@zzu.edu.cn
Received: 2006-08-28 Accepted: 2006-09-20
AbstractAIM:To establish a cisplatin-induced human esophageal carcinoma drug-resistant cell line Ec9706/cDDP and study its biological characteristics.
METHODS: The drug-resistant cell line Ec9706/cDDP was established in culture by exposing Ec9706 parent cells to moderate concentration of cisplatin (cDDP) over a period of 9 months ......
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