脂氧合酶抑制剂NDGA对乳腺癌MCF7细胞VEGF和CD44V6表达影响的研究
乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF,,乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF,1材料和方
【摘要】目的:探讨NDGA对乳腺癌MCF7细胞VEGF和CD44V6表达的影响。方法:将乳腺癌MCF7细胞系培养至对数生长期,分别以0、1、10、100μmol/L的NDGA处理48h后,RTPCR法检测细胞VEGFmRNA的表达水平,流式细胞仪检测细胞CD44V6的表达情况。结果:在1、10、100μmol/L的NDGA作用下,3组VEGFmRNA和CD44V6的表达显著低于对照组(F=2996.13,P<0.001;F=3435.08,P<0.001)。结论:NDGA明显下调MCF7细胞VEGFmRNA和CD44V6的表达,抑制肿瘤细胞的侵袭转移能力。【关键词】乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF
The effect of lipoxygenase inhibitor (NDGA) on the expression of VEGF and CD44V6 in breast cancer MCF7 cells
LIU Huantao,TANG Lubing,MA Rong
Department of Breast Surgery,Qilu Hospital of Shandong University(Jinan 250012,China)
【ABSTRACT】Objective:To explore the effects of VEGF and CD44V6 on breast cancer MCF7 cells by NDGA.Methods:MCF7 cells in the logarithmic growth state were selected and measured.There were four cell groups(concentration:0,1,10,100μmol/L)in the experiment.After 48h treatment of NDGA,the expression of VEGFmRNA was measured by RTPCR and the expression of CD44V6 was examined by flow cytometry.Results:After treatment of NDGA,the expressions of VEGFmRNA and CD44V6 in NDGA groups were significantly lower than that of the control group(F=2996.13,P<0.001;F=3435.08,P<0.001).Conclusion:NDGA can influence the invision and metastasis on MCF7 cells by means of the downregulated expression of VEGFmRNA and CD44V6. ......
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