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    WANG Yueª²Gang1, JIN Chunª²Hua2, HUANG Haiª²Xiao3, WU Pingª²Sheng1

    1Department of Cardiology, Nanfang Hospital, 2Center of Laboratory Administration, College of Basic Medicine, Southern Medical University, Guangzhou 510515, China, 3Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100853, China

    ¡¾Abstract¡¿ AIM: To explore the mechanism of polydatin (PD) treating shock through studying the influence of PD on the activity of protein kinase C (PKC) of vascular smooth muscle cells (VSMC) when treated by sera from burned rats and lipopolysaccharide (LPS). METHODS: After VSMC was treated by sera from burned rats or LPS, the activity of PKC was measured by ¦Ãª²scintillation counting instrument. RESULTS: Activity of PKC of VSMC cytoplasm after treated by sera from burned rats decreased (P<0.05 vs normal group), but activity of PKC of cell membrane increased (P<0.05 vs normal group). When VSMC was treated with PD, the activity of PKC of VSMC cytoplasm increased and that of cell membrane decreased (P<0.05 vs burned group and control group, P>0.05 vs normal group). However, the activity of PKC of VSMC cytoplasm and cell membrane treated by LPS increased (P<0.05 vs normal group), after PD treated VSMC the activity of PKC decreased again (P<0.05 vs LPS group and control group, P>0.05 vs normal group). CONCLUSION: PD antagonizes the damage effects of LPS and sera of burned rats by reversing the PKC activities, which may be one of the molecular mechanisms of PD antishock property. ......