麻疹_____麻疹病毒；核蛋白基因；序列分析；RTPCR

西安市麻疹野病毒核蛋白基因序列分析

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麻疹,,麻疹病毒；核蛋白基因；序列分析；RTPCR,1材料和方法,2结果,3讨论,【参考文献】

     SI Yuan1, LI Ping1, HOU TieJun2, SUN YaHui2, GUAN RongHui1, JI YiXin3, WANG Feng4

    1Shaanxi Provincial Center for Disease Control and Prevention, 2Xian Center for Disease Control and Prevention, Xian 710054, China, 3Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China, 4Department of Nutrition and Food Hygiene, School of Preventive Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the genotype and characteristics of measles virus found in Xian city. METHODS: Measles viruses were separated from the pharynx swabs of suspected measles patients within 3 d of rash emergence. The total RNA was isolated from the measles virus by the phenolchloroform extraction method. Then we amplified the 450 bp nucleotide segment of carboxylterminal region of nucleoprotein gene by RTPCR, determined the sequence of the amplified segments, analyzed and compared the sequence with those in Genbank. RESULTS: Eight strains of positive measles virus were isolated from 13 samples of pharynx swabs, the positive rate 62%. All of the isolated viruses were H1 genotype of measles virus. CONCLUSION: H1 genotype remains predominant strain of measles virus circulating in Xian city, which is consistent with the strain of measles virus circulating in China. ......

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