摘要:目的 研究Fas在人体大肠癌及癌旁组织中的表达。 方法 应用SP免疫组化方法(链霉菌抗生物素蛋白-过氧化物酶连接法)对49例大肠癌及癌旁组织中的Fas基因的表达进行检测。 结果 凋亡基因Fas在大肠癌组织中的阳性表达率高于癌旁组织(P<0.05)，且高分化腺癌组织中的阳性表达率高于癌旁组织(P<0.05)，但在不同分级的大肠癌及其癌旁组织之间的阳性率无明显差异。 结论 分化低的大肠腺癌癌旁组织中的细胞凋亡活动较活跃，有利于肿瘤的浸润与破坏。

    关键词:大肠癌;凋亡基因;Fas免疫组化

    The expression and significance of apoptotic genes Fas in large intestine cancer and neighboring tissues.

    ZHANG Ji-hong，SUN Bo，JIANG Jing，et al.

    (Affiliated Hospital of Beihua University，Jilin132001，Jilin，P.R.China)

    Abstract:Objective To investigate the expression of Fas in colonic cancer and near-by tissues. Methods The expression of Fas genes in colonic cancer and near-by tissues of49cases was determined by applying a method of SP immunohistochemical(streptomycin avidin-peroxidase connection). Results The results revealed that the positive expressive rate of apoptotic genes Fas in cilonic cancer was higher than in near-by tissues(P<0.05)and the expression rate in the tissuesof well-differentiated adenocar cinoma was also higher than that in the near-by tissues(P<0.05).The positive rate in colonic cancers of different grade was not significafltly different from that in their near-by tissues. Conclusion The apoptosis cells are more active in the near-by tissues of colonic adenocarcinoma of low-differentiation ......