摘要:目的 为了解人芽囊原虫对外界条件的适应性，观察培养条件改变对其形态和繁殖方式的影响。 方法 从腹泻患者粪便中检查并分离培养获得人芽囊原虫，分别用locke氏-鸡蛋双相培养基(LES)和改良1640培养基(RPMI-1640)培养，改变培养条件，观察人芽囊原虫形态和繁殖方式。 结果 在两种培养基中均可见到人芽囊原虫的四种主要形态:空泡型、颗粒型、阿米巴型和包囊型，其繁殖方式以二分裂为主，可见少量出芽生殖。当从LES培养基突然变为RPMI-1640培养基时，人芽囊原虫出现短时间(48～72h)内生长停滞，随后出现多种形式的繁殖方式，可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖，持续24h后恢复为二分裂为主。 结论 培养条件改变能够引起人芽囊原虫繁殖方式的改变，出现短暂性多样化繁殖方式，人芽囊原虫对环境的适应能力很强。

    关键词:人芽囊原虫;繁殖方式;多样化;暂时性;培养基

    Temporary coexistence phenomenon of diversified reproductive mode of Blastocystis hominis.

    ZHANG Hong-wei，HE Li-jun，LI Wen，et al

    (Henan Provincial Center for Disease Control and Prevention，Zhengzhou450003，Henan，P.R.China)

    Abstract:Objective To understand the adaptability of Blastocystis hominis(B.hominis)to environment，observe effect of culture condition change on reproductive mode of B.hominis. Methods B.hominis was isolated from patient with diarrhea and cultured in different culture medium including Locke-egg-serum medium culture(LES)and modified RPMI-1640culture medium(RPMI-1640).When culture condition was changed between LES and RPMI-1640，morphous and reproductive modes were ob-served. Results All of4form including vacuolar form ......