摘要:目的 构建以结核分枝杆菌热休克蛋白65和汉坦病毒G2重组融合基因为基础的核酸疫苗。 方法 采用聚合酶链反应从结核杆菌H37Rv株基因中，扩增出HSP65的编码基因，从T-H8205G2质粒扩增出G2蛋白的编码基因，经相应限制性核酸内切酶消化后，插入真核表达载体pcDNA3.1/His，并将此重组质粒通过酶切、SDS-PAGE分别测定表达产物的相对分子量。 结果 获得正向插入的pcDNA3.1/His-HSP65-G2重组表达质粒，与理论预期大小一致。 结论 以编码HSP65和G2抗原基因的重组基因的真核表达载体构建成功，为进一步研究其在结核病和出血热防治中的作用奠定了基础。

    关键词:核酸疫苗;结核分枝杆菌;热休克蛋白65;汉坦病毒;G2蛋白

    Construction and recombinant of hsp65gene of Mycobacterium tuberculosis and G2gene of Hantavirus H8205.

    HUANG Hao，HUANG Han-ju，GUAN Jiang-feng，et al.

    (Central Laboratory Department of Wuhan Municipal First Hospital，Wuhan430022;Department of Pathogenic Biology，Tongji Medical College Elementary School of Huazhong Science and Technology Un-versity，Wuhan430031，Hubei，P.R.China)

    Abstract:Objective To construct nucleotide vaccine based on the recombinant plasmids of hsp65gene of mycobacterium tu-berculosis H37Rv strain and G2gene fragments of Hantavirus H8205strain. Methods The gene encoding heat shock protein65kd and G2glycoprotein were amplified from genome of Mycobacterium tuberculosis and T-H8205G2plasmid by routine polymerase chain reaction(PCR).After corresponding to restriction endonuclease digestion ......