摘要:目的 探讨已酮可可碱(pentoxifylline，PTX)对大鼠全脑缺血再灌注后炎症反应的影响及其治疗作用。 方法 采用四血管阻塞的方法，复制出大鼠全脑缺血再灌注模型。采用免疫组化法、原位杂交法和原位末端标记法分别检测假手术组(A组)、全脑缺血再灌注组(B组)、已酮可可碱治疗组(C组)海马CA1区核因子κB(NF-κB)、肿瘤坏死因子-αmRNA(TNF-αmRNA)及细胞凋亡数的变化。 结果 与假手术组比较，全脑缺血再灌注组海马CA1区NF-κB和TNF-αmRNA的表达及细胞凋亡数明显增加(P<0.01);NF-κB和TNF-αmRNA的表达分别于再灌注6h和12h达到高峰，并持续到48h;细胞凋亡数随再灌注时间的延长而逐渐增多(P<0.01)。PTX治疗后，NF-κB和TNF-αmRNA的表达下降，细胞凋亡数减少(P<0.01)。 结论 在脑复苏救治过程中，PTX可抑制NF-κB与TNF-αmRNA的表达，抑制炎症反应，减少细胞凋亡而发挥脑保护作用。

    关键词:已酮可可碱;全脑缺血再灌注;核因子-κB;肿瘤坏死因子-αmRNA;凋亡

    Effect of pentoxifylline on inflammatory reaction of rats with global cerebral ischemia-reperfusion.

    CHEN Wei-feng，LIU De-hong.

    (Emergency Department of Shenzhen Municipal Second People′s Hospital，ShenZhen518028，Guangdong，P.R.China)

    Abstract:Objective To explore the protective effects of pentoxifylline(PTX)on rats with global cerebral ischemia-reperfu-sion. Methods The model of global cerebral ischemia-reperfusion was established by four-vessel occlusion method using im-munohistochemical technique，in situhybridization and in situ end labeling technique(TUNEL)，the expressions of NF-κB and TNF-αmRNA and the number of the apoptotic cell were examined in the sham-operated group(group A) ......