Antiinflammatory profiles during primary SIV infection in African green monkeys are associated with protection against AIDS
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《临床调查学报》
1Unite de Biologie des Retrovirus, Institut Pasteur, Paris, France.
2Centre International de Recherche Medicale, Franceville, Gabon.
3Laboratoire de Retrovirologie, Institut Pasteur, Dakar, Senegal.
4 Unite de Recherche et d’Expertise Physiopathologie des Infections Lentivirales, Institut Pasteur, Paris, France.
5INSERM 567, UMR CNRS 8104, IFR 116, Departement d’Immunologie, Institut Cochin, Paris, France.
6 Universite Paris VII Denis Diderot, Paris, France.
7Commissariat a l’Energie Atomique, Fontenay aux Roses, France.
Abstract
T cell activation levels in HIV infection are predictive of AIDS progression. We searched for the immunological correlates of protection against disease progression by studying the early stages of nonpathogenic SIV infection in African green monkeys (SIVagm). The African green monkeys (AGMs) displayed high peak viremias and a transient decline in levels of blood CD4+ and CD8+ T cells between days 5 and 17 after infection. A concomitant increase in levels of CD4+DR+, CD8+DR+, and CD8+CD28– cells was detected. After the third week, T cell activation returned to baseline levels, which suggested a protective downregulation of T cell activation. A very early (24 hours after infection) and strong induction of TGF-?1 and FoxP3 expression was detected and correlated with increases in levels of CD4+CD25+ and CD8+CD25+ T cells. This was followed by a significant increase in levels of IL-10, whereas IFN- gene upregulation was more transient, and levels of TNF- and MIP-1/? transcripts did not increase in either blood or tissues. The profiles were significantly different during primary SIV infection in macaques (SIVmac); that is, there was a delayed increase in IL-10 levels accompanied by moderate and persistent increases in TGF-? levels. Together, our data show that SIVagm infection is associated with an immediate antiinflammatory environment and suggest that TGF-? may participate in the generation of Tregs, which may prevent an aberrant chronic T cell hyperactivation.
Introduction
HIV-1 infection is characterized by chronic T cell hyperactivation. Increased T cell activation from the initial stage of infection has predictive value for AIDS progression even before seroconversion and independently of viral load (VL) (1-4). The precise mechanisms leading to this aberrant chronic T cell activation remain unclear. The study of SIV infections in nonhuman primate models has contributed to an understanding of the mechanisms underlying CD4+ T cell depletion and AIDS. The animal model that best reflects HIV infection in humans is SIV infection in macaques (SIVmac). SIV infections are generally only nonpathogenic in African nonhuman primate species, the natural hosts of SIV, such as African green monkeys (AGMs), mandrills (MNDs), sooty mangabeys (SMs), and chimpanzees (CPZs) (5). During SIVagm, SIVsm, SIVmnd-1, and SIVcpz infections in their respective hosts, VLs have been shown to be similar to those recorded for pathogenic HIV-1 and SIVmac infections (6). Furthermore, SIVagm replicates in lymphoid tissues, including the gut (6, 7). Thus, these studies have shown that viral replication alone is not sufficient to drive CD4+ T cell decline.
Despite high levels of VLs, natural hosts show distinct lymphocyte activation profiles during chronic infection compared with humans and macaques. Naturally infected AGMs and SMs do not exhibit significant LN follicular hyperplasia (8, 9) or infiltration of CD8+ cells into LN germinal centers (9). Naturally infected MNDs and SMs exhibit nearly unchanged percentages of CD4+DR+ T cells and only slight increase in the percentages of CD8+DR+ T cells in blood (10, 11). Long-term SIVsm infection in SMs is characterized by normal T cell turnover and decreased IFN- expression that correlates with low CTL activity (8, 11, 12). Finally, chronically infected AGMs, SMs, and CPZs failed to show enhanced peripheral CD4+ T cell apoptosis levels (11, 13). Data on T cell activation and cytokine profiles during the initial stage of SIVagm in AGMs are crucially needed in order to understand the very early events that will shape immune responses and prevent the aberrant chronic T cell activation despite continuous viral replication and the following questions must be addressed: (a) Are the T cells activated during primary infection in response to SIVagm? (b) Are the cytokine profiles pro- or antiinflammatory? (c) Is the low activation associated with tolerance toward SIVagm?
To address these questions, we studied T cell activation patterns in distinct tissues of SIVagm-infected AGMs during primary infection. CD4+ and CD8+ T cell subpopulations were examined in peripheral blood as well as in secondary lymphoid organs. To analyze the early orientation of the immune response, we compared the cytokine profiles of 3 distinct compartments — blood (i.e., at a systemic level), LN, and peripheral tissue (by bronchoalveolar lavage [BAL] — at initiation and effector sites of the immune response. This study reveals that SIVagm infection in AGMs can lead to a transient T cell activation during primary infection but that AGMs mount an immediate antiinflammatory cytokine environment. This raises the possibility of a Treg induction, which may then control T cell turnover rates.
Results
Transient decline in blood CD4+ T cells during primary SIVagm infection.
Nine AGMs infected with SIVagm.sab92018 as well as 2 control animals were included in this study. To allow comparison between pathogenic SIVmac and nonpathogenic SIVagm infection under the same experimental conditions, we included 2 SIVmac251-infected macaques as well as 2 control macaques as internal control for phenotypic analysis of CD4+ and CD8+ T cells (Figures 1). Both infected macaques progressed rapidly toward disease, developing AIDS within 7 months after infection. Plasma VLs were very similar in SIVmac-infected macaques and SIVagm-infected AGMs (Figure 1). VLs peaked between 6 and 10 days after infection (3 x 107 to 8 x 107 copies/ml of plasma). After day 35, the plasma VLs ranged from of 2 x 103 to 8 x 104 copies/ml and were within the range previously recorded by us and others in chronically infected AGMs and macaques (6, 14, 15).
We then compared the early T cell dynamics in macaques and AGMs. In both SIVmac-infected macaques (MACQ172 and MACQ205), the blood CD4+/CD8+ T cell ratios decreased starting from day 10 after infection. (Figure 2A). After day 10, the absolute number CD4+ T cells remained low compared with baseline, whereas numbers of CD8+ T cells returned to baseline (data not shown). In AGMs, an early significant decline was also detected, reaching a maximum between days 7 (P < 0.0001) and 10 (P < 0.0001) (Figure 2B). This decline was due to a more pronounced decrease in the absolute number of CD4+ T cells compared with CD8+ T cells (data not shown). Both CD4+ and CD8+ T cell counts reached preinfection values after day 10 and remained stable. There was a negative correlation between plasma SIVagm RNA levels and CD4+ T cell counts ( = –0.541; P < 0.0001) during primary SIVagm infection. Together, our results indicate that primary SIVagm infection in AGMs, like pathogenic SIVmac infections in macaques, can lead to a significant decrease in the absolute number of CD4+ T cells in blood.
To determine whether modulations in blood T cell subsets are reflected in secondary lymphoid tissues, we analyzed CD4+ and CD8+ T cell percentages in LN during SIVagm and SIVmac infections. Whereas the 2 mock-infected control macaques had a stable CD4+/CD8+ T cell ratio in LN throughout the experimental follow-up, both SIVmac-infected macaques exhibited a decrease during the acute phase of the infection. This decline was more pronounced during the chronic phase of infection (Figure 2C). By contrast, SIVagm-infected AGMs showed no significant changes in CD4+/CD8+ T cell ratios within LN at any time during the period of observation (Figure 2D). This observation is consistent with our previous finding of a lack of CD8+ T cell infiltrations into LN germinal centers (9).
Transient increases of activated CD4+ and CD8+ T cells in blood during primary SIVagm infection.
To determine whether decreases in circulating CD4+ and CD8+ T cells in AGMs were associated with changes in T cell activation levels, we performed a comparative analysis of T cell activation markers in blood and LN cells during primary SIVagm and SIVmac infection. We focused our comparative analysis on 2 cell-surface markers (DR and CD28), whose expression levels correlate with disease progression in HIV-1/SIVmac infections (1, 3, 16).
We analyzed T cell activation in macaques and AGMs. None of the SIVmac-infected macaques studied showed significant changes in the proportion of circulating DR-expressing CD4+ T cells during primary infection, but the proportion was enhanced after day 28 (Figure 3A). By contrast, in AGMs, there was a significant increase in the percentages of CD4+DR+ cells only at early time points, i.e. at days 7 (P = 0.003) and 10 (P < 0.0001) (Figure 3B). Activation profiles within the circulating CD8+ T cell population mirrored those observed for circulating CD4+ T cells. During primary SIVmac infection in macaques, an early, slight increase in CD8+DR+ T cells was followed by a persistent increase during chronic infection (Figure 3C) concomitantly to a continuous decrease of CD8+CD28+ T cells (data not shown). In AGMs, the baseline percentage of DR+ peripheral blood CD8+ T cells was more variable than that seen in macaques (ranging from 8% to 62%). After SIVagm infection, the percentages of CD8+DR+ T cells were significantly increased between days 8 and 21 (P 0.044 for each) (Figure 3D). Values then declined to baseline levels by day 28. AGMs also exhibited significant decreases in CD8+CD28+ T cells between days 5 and 21 (P 0.035 for each) (data not shown). After day 21, the percentages of CD8+CD28+ T cells returned to their preinfection values. We thus confirm a lack of increased T cell activation during the chronic phase of infection and reveal for the first time to our knowledge that T cell activation can occur during primary SIVagm infection.
The percentages of activated CD8+ T cells in AGM LNs remain unaltered after SIVagm infection.
There was no significant change in the frequency of LN CD4+DR+ and CD4+CD28+ T cells from macaques and AGMs after infection (data not shown). An increase in CD8+DR+ and CD8+CD28– T cells was observed after 60 days in the SIVmac-infected macaques, whereas in AGMs, no significant changes were evidenced in LN (data not shown). During the chronic phase of infection, there is therefore, as expected, a lower level of CD8+ T cell activation in the LN of AGMs compared with macaques. That the frequencies of DR- and CD28-positive CD8+ T cells in LN were unaltered during primary infection contrasts with the changes detected in the periphery but is consistent with the absence of CD4+/CD8+ T cell ratio changes.
Expression of RANTES, CCR1, and CCR5 transcripts is transiently upregulated in acutely SIVagm-infected AGMs.
The presence of higher levels of activated T cells and of infiltrating cells in LN in pathogenic infections may result from a heightened state of inflammation. We searched for differences in the early pro- and antiinflammatory cytokine profiles induced during primary infection. We analyzed the profiles in AGMs in 3 distinct compartments: PBMCs, LN lymphocytes (LNLCs), and BAL cells (BALCs), a peripheral compartment that has been found to present signs of inflammation in acutely SIVmac-infected macaques (17, 18). The 3 compartments were studied in 5 AGMs (3 randomly selected SIVagm-infected AGMs and the 2 mock-infected control AGMs). We first measured ? chemokine expression as a marker for inflammation.
During primary SIVagm infection, RANTES gene expression was significantly increased (at days 7, 10, 17, 21, and 30 in PBMCs (P 0.04 for each) and in BALCs from days 7 to 30 (P 0.027 for each). In LN, increases in RANTES expression levels were observed in both SIV- and mock-infected animals and were thus not considered to be specifically induced by SIVagm infection (Figure 4, A and B). Changes in the expression levels of MIP-1 and MIP-1? genes were less frequently observed in infected animals than for RANTES and/or also observed in the mock-infected AGMs (Figure 4, A and B) after infection and throughout acute infection (P 0.027 for each). In summary, these observations demonstrate that SIVagm infection in AGMs leads to induction of RANTES, CCR1, and CCR5 gene expression in PBMCs and BALCs, whereas no infection-specific increases were detected in LN. Furthermore, no or only very modest gene expression changes of MIP-1 and MIP-1? were detected.
Increased IL-10/TNF- ratios during primary SIVagm infection.
To further explore the possibility that proinflammatory cytokine expression is dampened during SIVagm infection, we analyzed the gene expression levels of TNF- and IL-10. Their expression was first measured in the 3 tissue compartments of the same 5 AGMs analyzed for chemokine gene expression (Figure 5), as well as in the 6 other AGMs at earlier time points with shorter intervals in PBMCs (Figure 6). TNF- gene expression was increased in PBMCs at days 5, 10, and 17 after infection (P < 0.042 for each) and in LN at days 5 (P = 0.008) and 21 (P = 0.007), but mock-infected control AGMs also exhibited similar TNF- gene expression changes in both PBMCs and LN (Figure 5). The increases in TNF- expression therefore appear not to be SIV specific but rather due to other factors, such as repeated sampling and/or injection of plasma. In BALCs of both SIVagm- and mock-infected AGMs, no significant changes in TNF- gene expression were detected at any time during the study period (Figure 5B), in contrast to what has been observed in macaques (19). The expression of the IL-10 gene in PBMCs and BALCs from SIVagm-infected AGMs (Figure 5A), but not mock-infected controls (Figure 5B), was significantly increased starting from day 7 until day 30 after infection (P < 0.05 for each). In LN, IL-10 gene expression was significantly increased at days 14 (P = 0.043) day 21 (P = 0.008) in infected AGMs and significantly increased at day 14 (P = 0.043) in mock-infected animals.
In summary, the above studies indicated TNF-/IL-10 ratios in favor of IL-10, specially in PBMCs and BALCs. We extended our analysis of TNF- and IL-10 gene expression in PBMCs to the 6 other AGMs. The kinetics of sampling were similar to those carried out above, except that we also collected blood at earlier time points (days 1 and 3 after infection), since the inflammatory balance might be established very early. The 6 AGMs also exhibited the same TNF-/IL-10 ratio in favor of IL-10 in PMBC (Figure 6A).
To further confirm this antiinflammatory cytokine environment in response to SIVagm infection, we measured TNF- and IL-10 protein concentrations in plasma. Plasma TNF- in all 6 SIVagm-infected AGMs measured throughout the acute phase and at day 120 always remained under the detection limit of the assay (15 pg/ml) (data not shown). In contrast, we detected heightened levels of IL-10 in plasma during primary infection, and the increases were statistically significant at days 6, 8, 10, and 13 (P 0.002 for each) (Figure 6B). In order to confirm that the early antiinflammatory cytokine environment was specific for SIVagm infection, we assayed IL-10 protein concentrations in 6 SIVmac251-infected rhesus macaques. They were significantly decreased from days 1 to 10 (P 0.019 for each) and significantly increased only later on day 21 (P = 0.024) (Figure 6B). These data support the previous conclusion that an antiinflammatory environment is induced early in SIVagm infection compared with pathogenic SIVmac infection.
Transient IFN- gene expression increases during primary SIVagm infection.
IL-10 has been implicated in the suppression of Th1-type immune responses. We therefore followed the changes in the gene expression of a typical Th1-type cytokine, IFN-. We first compared IFN- gene expression in the 3 tissue compartments of the 3 SIVagm-infected and 2 mock-infected AGMs. In LNLCs and BALCs, no statistically significant changes in IFN- gene expression were detected at any time after SIVagm infection (Figure 5). In PBMCs, the SIVagm-infected animals demonstrated a significant increase at days 5 (P = 0.018) and 10 (P = 0.033). In the 6 other AGMs, we also observed a similar transient increase in PBMCs at days 10 (P = 0.005) and 16 (P = 0.019) (Figure 6A). To summarize, the analysis of IFN- gene expression detected increases only in PBMCs, but not in LNLCs and BALCs, in contrast to IL-10. In PBMCs, the increases in IFN- gene expression appeared to be more transient than those in IL-10 expression.
Immediate increases of CD4+CD25+ and CD8+CD25+ T cells in blood.
The above data reveal that primary SIVagm infection in AGMs is associated with a transient activation of T cells, TNF-/IL-10 ratios in favor of IL-10, and a weak IFN- response. These observations raised the hypothesis that Tregs are induced after SIVagm infection of AGMs. To evaluate this possibility more closely, we analyzed the frequency of CD4+CD25+ and CD8+CD25+ T cells in 6 AGMs from time points directly after infection (day 1) through day 430 after infection.
At days 1 and 6, percentages of CD4+CD25+ and CD8+CD25+ T cells in peripheral lymphocytes were significantly increased (P 0.014 for each) (Figure 7, A and B). At days 13, 16, and 21, percentages of CD8+CD25+ T cells rebounded (P 0.038 for each) after a decrease at days 8 and 10 (P 0.0005 for each), and throughout the chronic phase, the percentages of CD4+CD25+ and CD8+CD25+ T cells were significantly lower (P 0.026 for each) relative to preinfection levels (Figure 7, A and B). A similar pattern was observed for the absolute counts of CD4+CD25+ and CD8+CD25+ T cells, the percentages of CD4+CD25+ in CD4+ T cells, and the percentages of CD8+CD25+ in CD8+ T cells (data not shown).
Immediate increases of TGF-? 1 and FoxP3 gene expressions during SIVagm.
CD25 is not a specific marker of Tregs, as it is also upregulated upon conventional T cell activation. Recently, a transcription factor (FoxP3) has been described to be specifically expressed in humans by thymic and peripheral regulatory CD8+ and CD4+CD25+ T cells (20, 21). To determine whether the increased levels of CD4+CD25+ and CD8+CD25+ T cells observed in SIVagm-infected AGM were Tregs or conventional activated T cells, we quantified FoxP3 transcript levels in PBMCs of the same 6 animals. We found significantly increased FoxP3 levels at days 1 and 6 after infection (Figure 7C). Correlations between both numbers and percentages of CD4+CD25+ T cells and FoxP3 gene expression trended toward significance ( = 0.280, P = 0.076 and = 0.278, P = 0.079, respectively). A statistically significant positive correlation was observed between FoxP3 gene expression and both the numbers ( = 0.548, P = 0.0003) and percentages of CD8+CD25+ T cells ( = 0.554, P = 0.0002).
It has been previously found that FoxP3 gene expression and subsequent conversion of naive CD4+CD25– T cells into CD4+CD25+ Tregs is induced by TGF-?1 (22). Accordingly, we monitored TGF-?1 gene expression in PBMCs during SIVagm infection. We observed statistically significant increased levels of TGF-?1 (Figure 7D). The changes were observed very early at days 1, 3, and 13–16 after infection (P = 0.0008, P = 0.004, and P = 0.028, respectively) and there was a trend toward significance at day 6 (P = 0.079). The levels of FoxP3 and TGF-?1 gene expression were highly correlated ( = 0.863, P < 0.0001). Interestingly, immediate increases (day 1 after infection) were only observed for FoxP3 and TGF-?1, whereas the expression of other cytokines, such as IL-10, was only increased several days later during primary infection. According to covariance analysis, TGF-?1 gene expression had a significant effect after SIVagm infection on gene expressions of the other cytokines (P = 0.044, multivariate analysis of covariance [MANCOVA]), whereas IL-10 expression did not have a significant effect (P = 0.72, MANCOVA). TGF-?1 gene expression did not correlate with the numbers of CD4+CD25+ T cells ( = 0.251, P = 0.112) but did correlate with the percentages of CD4+CD25+ T cells ( = 0.372, P = 0.019) and, as had FoxP3, with both numbers ( = 0.557, P = 0.0002) and percentages of CD8+CD25+ T cells ( = 0.554, P = 0.0002).
To confirm the increases of TGF-?1 gene expression at the protein level, we assayed TGF-?1 in plasma during primary SIVagm infection. Our results clearly revealed a statistically significant increase in plasma TGF-?1 at day 1 after infection (P < 0.0001) (Figure 7E). Then plasma concentrations returned to baseline levels at days 3 and 6 (except for 2 of the 6 animals at day 3 [SAB97008 and SAB98013 and at day 6 [SAB01015 and SAB00020, for which concentrations of plasma TGF-?1 remained higher [2- to 3-fold] compared with their preinfection levels). Throughout days 10–21, concentrations of plasma TGF-?1 were lower than preinfection baseline levels (P < 0.0001). Again, we found that TGF-?1 concentrations in plasma were significantly correlated with the absolute numbers of CD8+CD25+ T cells in blood ( = 0.375, P = 0.033). This early rise of TGF-?1 concentrations in plasma is consistent with our previous conclusion that an antiinflammatory cytokine environment is immediately induced after SIVagm infection.
In order to study whether or not the early TGF-?1 increase is present during primary pathogenic SIVmac infection, we assayed TGF-?1 in plasma from the 6 SIVmac251-infected rhesus macaques for which we had assayed IL-10 concentrations in plasma. We observed significant increases at days 1, 3, 6, 10, 13, and 16 (P 0.043 for each) (Figure 7F). Nevertheless, the increases at day 1 after SIVmac infection were significantly lower compared with those after SIVagm (P = 0.037, Mann-Whitney U test). Interestingly, the profiles were inversed after VL peaked (days 10–21). Indeed, TGF-?1 concentrations in infected macaques were significantly higher than those in infected AGMs at days 10 and 16 (P = 0.037 and P = 0.025, respectively, Mann-Whitney U test). Therefore, the patterns were different in pathogenic and nonpathogenic infections, with an immediate strong increase in TGF-?1 levels after primary SIVagm infection and a moderate persistent increase after primary SIVmac infection.
Discussion
The present study on nonpathogenic SIVagm infection adds significantly to our understanding of critical factors involved in AIDS protection. Several mechanisms have been proposed as driving progression toward AIDS in humans and macaques: (a) accelerated cell death, (b) impaired T cell generation, and (c) accelerated turnover leading to exhaustion. Our results show that during primary SIVagm infection in its natural host, a decline in peripheral CD4+ T cells to levels similar to those found in pathogenic SIVmac can occur. The CD4+ T cell decline was slightly greater than that of CD8+ T cells and may thus partially result from virus-induced cell death. SIVagm has indeed been shown to be as cytopathic as HIV-1 or SIVmac in vitro (23). However, since CD8+ T cells also decrease, albeit to a lesser extent than CD4+ T cells, an additional reason for loss of T cells in the periphery could be bystander cell death and/or migration to LN. Bystander cell death of CD8+ T cells has been demonstrated in several natural hosts, including AGMs (13). Although we did not see any changes in the CD4+/CD8+ T cell ratio in LN, a slight, equal increase in both CD4+ and CD8+ T cells cannot be excluded. If such an increase did occur, it must be moderate in comparison with that in SIVmac-infected macaques, since no lymphadenopathy has been evidenced in LN of SIVagm-infected AGMs (9). The detection of increased percentages of activated CD8+ T cells during primary infection in blood but not in LN of AGMs might be explained by a release of activated cells in the circulation in AGMs. In contrast, the higher and more persistent levels of activated T cells in LN in macaques suggests a sequestration of activated cells in LN during pathogenic HIV-1/SIVmac infections. This hypothesis is consistent with the lack of infiltration of CD8+ cells in germinal centers of LN of infected AGMs (9).
The lack of chronic hyperactivation seems indeed to be a common feature of nonpathogenic SIV infections in natural hosts (8-11, 13, 24). In HIV infection, evidence has been accumulating to support the concept that the degree of activation is the best predictor of disease progression, whereas VL is only indirectly associated with progression (1, 2, 4). CD8+ T cell activation at early stages in HIV infection is determinant of the rate at which CD4+ T cells are lost over time (3). Thus, it is critical to identify the mechanism(s) that protect against the harmful hyperactivation of the immune system. It has been proposed that protection in AGMs could be due to tolerance against a SIVagm protein (25). Our study reveals a state of increased T cell activation in peripheral blood subsequent to SIVagm infection, which thus clearly demonstrates that AGMs respond to SIVagm infection and that protection is not due to a central tolerance mechanism. However, our data on IFN- gene expression suggest a low Th1 response. We found no increase in LNLCs and BALCs and increases in PBMCs only between days 5 and 16 after infection. This is consistent with the decreased IFN- production in CD4+ T cells and low CTL frequencies reported in chronically SIVsm-infected SMs (11, 12). It is beneficial to maintain a Th1-type cytokine secretion pattern when it is associated with efficient CTL responses during chronic HIV-1 infection, as observed in some LTNPs (26). Although, Th1 responses are protective, they may be harmful if they are associated with strong, generalized immune activation (12, 27). An et al. indeed reported that a TNF-–inducible promoter variant of IFN- (–179T) accelerates loss of CD4+ T cells in humans (28). In clinical trials with IFN-, both acceleration of AIDS progression and decline of CD4 cell counts have been reported (29). Recently, it has been shown that the long-term challenge outcome in vaccinated macaques was correlated with the relative balance between SIV-specific IFN- T cell responses and nonspecific IFN-–driven inflammation, the latter being seen in animals in which viral replication cannot be controlled (30).
We found that an antiinflammatory environment is established early during SIVagm infection. Our data on ? chemokine expression are in contrast to those on HIV-1 infection in humans or SIVmac-infected macaques, where significant increases of RANTES, MIP-1, MIP-1?, and CCR5 gene expressions have been observed in PBMCs, LN, and BALCs (17, 18). Recently, it has been reported that high ? chemokine expression levels in lymphoid tissues of SIVmac-infected macaques contribute to nonspecific inflammation and immune activation as well as to high VL in lymphoid tissues (31). RANTES is chemotactic mainly for monocytes and CD4+ memory T cells, whereas MIP-1 and MIP-1? have been shown to attract CD8+ T cells (32). Therefore, the lack of MIP-1 and MIP-1? gene overexpression in SIVagm infection might be associated with the lack of CD8+ lymphocytosis and CD8+ infiltration in LN germinal centers (9).
SIVagm-infected AGMs also exhibited no signs of significant TNF- increases. This contrasts with TNF- increases detected in acutely SIVmac-infected macaques and in HIV-infected humans (14, 33, 34). We previously showed, for instance, that SIVmac-infected cynomolgus macaques produce up to 1,500 pg TNF-/ml of serum during primary infection (35). The lower TNF- expression during SIVagm infection was not due to a constitutive impairment, since we detected TNF- (up to 100 pg/ml) after ConA stimulation of AGM mononuclear cells (data not shown). Rather, as shown here, AGMs exhibit a significant increase of IL-10 during primary infection. Although increased IL-10 expression levels have been associated with disease progression during later stages of HIV-1 infection (36), a higher stimulus-induced IL-10 mRNA expression, together with a lower constitutive TNF- mRNA expression — similar to what we observed in AGMs — were indeed associated with slower disease progression in HIV-1-infected children (37). Moreover, a mutation in the IL-10 promoter region, resulting in constitutively reduced IL-10 gene expression, is associated with a more rapid progression toward AIDS in HIV-1–infected patients (38). One explanation for the discrepancy in the findings on the role of IL-10 might thus be related to the different disease stages studied. Here our data show that during acute SIVmac251 infection in rhesus macaques, IL-10 secretion in plasma was indeed significantly increased, but this occurred later than in nonpathogenic SIVagm infection. Previous studies on SIVmac-infected macaques showed enhanced levels of IL-10 gene expression in PBMCs in a few animals during primary SIVmac infection, but this expression was generally accompanied by strong TNF- gene expressions (14, 19). Together, our data reveal early induction of an antiinflammatory environment in response to SIVagm infection in AGMs. This may be what determines the subsequent low, generalized immune activation levels.
This antiinflammatory environment might be induced in AGMs by TGF-?1, whose expression is upregulated as early as 24 hours after infection to levels significantly higher than those in macaques, which show greater levels only starting at day 10. Omer et al. observed that in Plasmodium yoelii–infected mice, a burst of TGF-?1 within 24 hours inhibits IFN- and TNF- responses, whereas increases of TGF-?1 4 days later do not (39). Moreover, the environment in which TGF-?1 is produced starting from the second week after infection is also different between pathogenic and nonpathogenic SIV infections, since in macaques it is concomitant with a proinflammatory cytokine balance (14, 17-19, 33, 35). The response by TGF-?1 that is weaker early and stronger thereafter in macaques may reflect an inability to prevent a fatal overwhelmed immune response and a continuous need for tissue repair mechanisms, as conferred by TGF-?1 (40), due to an inability to resorb virus-driven inflammation. In Plasmodium yoelii–infected mice, the major producers of TGF-?1 are adherent cells, CD8+ and CD25+ cells (39). In AGMs, we found that the most statistically significant correlation of TGF-?1 (transcript and plasma levels) was with CD8+CD25+ T cells. It remains to be determined which cells express TGF-?1 during primary nonpathogenic SIVagm infection and pathogenic SIVmac infection, but CD8+CD25+ T cells could be one of the major sources of TGF-?1 during primary SIVagm infection.
TGF-?1 might be responsible for the induction of FoxP3, which correlates with the increases of CD25+ cells, in particular CD8+CD25+ T cells. Although the presence of Tregs in AGMs needs to be confirmed by functional studies, the hypothesis that they are induced is strongly supported by our findings. So far, it is unclear whether they would correspond to natural (Trn) or to peripherally induced (Tr1, TH3) Tregs or both. One cannot exclude the possibility that a first expansion of FoxP3+ Trn might enhance the induction of peripheral IL-10–producing FoxP3– Tregs, eventually associated with the repeated Ag stimulations in the absence of proinflammatory signals (41). Further studies are needed to determine to which subset of CD8+ and/or CD4+ Tregs the cells might belong.
Such an induction of Tregs could explain the downregulation of T cell activation in AGMs. The presence of CD4+ and CD8+ Tregs has been described ex vivo during the chronic phase in HIV-1–infected patients (42-46). Some of these studies proposed that an impaired generation of CD4+CD25+ Tregs might be at the origin of the uncontrolled T cell hyperactivation (44, 45), whereas others proposed that Tregs could be at the origin of immunosuppression in HIV-1 infection (42, 46). Thus, it is not clear so far whether they play a protective role against T cell hyperactivation or rather contribute to immunosuppression in HIV infection. Our data are in favor of a protective role of Tregs. We and others have previously shown that primary SIVmac infection is associated with increases in CD4+CD25+ T cells in blood between days 7 and 21 after infection but with unchanged CD8+CD25+ T cell levels (47, 48). One can hypothesize that SIVmac-infected macaques exhibit chronic abnormal T cell activation due to a delayed expansion and/or a lack of CD8+ Treg induction.
In summary, our study describes, for the first time to our knowledge, the balance between the expression of pro- versus antiinflammatory cytokines within the first days after infection during nonprogressive SIV infection, and suggests a mechanism for avoiding harmful generalized immune activation after lentiviral infection. Our data reveal an immediate antiinflammatory balance in AGMs in response to SIVagm infection. This balance might be primed by TGF-?1, which is induced early and strongly and which might lead to the very early increase (starting from day 1 after infection) of FoxP3 expression. The potential rapidly induced CD4+ and CD8+ Tregs might temper the proinflammatory cytokine cascade and protect against chronic hyperactivation. These new insights into the early cytokine balance and potential protective role of Tregs are crucial for the research on novel immune intervention strategies for the prevention and treatment of AIDS.
Methods
Viral stocks.
The generation and titration of the SIVagm.sab92018 virus stock has been described elsewhere (9). The cell-free SIVmac251 stock was kindly provided by A.M. Aubertin (Universite Louis Pasteur, Strasbourg, France). We used AGMs inoculated with SIV-negative plasma as mock-infected controls. This plasma was collected from the same monkey from which samples were used to prepare the SIVagm stock. The negative control plasma for macaques was obtained from an unrelated donor macaque.
Animals and infections.
AGMs used in these studies belonging to the Chlorocebus sabaeus species were housed at the Pasteur Institute facility in Dakar, Senegal. Cynomolgus macaques (MAC141A, MACN894, MACQ172, and MACQ205) were housed at the Centre International de Recherche Medicale in Franceville, Gabon. Chinese rhesus macaques (MAC1058, MAC1073, MAC1076, MAC1085, MAC1094, and MAC1099) were housed at the Pasteur Institute facility in Ile-de-France, France. All animals were housed according to national guidelines and institutional policies. Animal care and use were approved by the Committee for Ethics and Animal Experimentation at the International School of Science and Veterinary Medicine in Dakar, Senegal. For infections, 100 and 300 TCID50 (where TCID50 is the tissue culture infective dose that affects 50% of inoculated cultures) SIVagm.sab92018 were intravenously inoculated into 3 (SAB98007 SAB98008 and SAB98011 and 6 (SAB97008 SAB98013 SAB00020 SAB01013 SAB01015and SAB02003 sabaeus monkeys (a sub-species of AGMs), respectively. The 3 cynomolgus macaques were intravenously inoculated with 100 TCID50 of SIVmac251. The 6 rhesus macaques were intravenously inoculated with 10 AID50 (where AID50 is the animal infective dose that affects 50% of inoculated cultures) of SIVmac251. Four control animals (2 sabaeus and 2 cynomolgus macaques) were intravenously inoculated with the corresponding volume (0.03–1 ml) of species-specific SIV-negative plasma. Although, we cannot exclude the possibility that differences between SIVagm.sab92018 and SIVmac251 infections described here are virus dependent, we used models in which monkeys were infected with their species-specific SIVs.
Specimen collection.
For every monkey included in the protocol, 5 ml of whole blood was collected in EDTA K2 tubes. PBMCs were isolated by Ficoll-Hypaque (Sigma-Aldrich) gradient centrifugation, after which plasma was recovered and clarified from cellular debris and residual platelets by additional centrifugation at 3,000 g for 5 minutes.
Excisional axillar and inguinal LN biopsies were performed, and tissues were mechanically dissociated. LNLCs were separated from the remaining tissue by passage through a 100-mm nylon mesh. A total of 2 x 106 fresh cells were used for flow cytometry.
BAL was carried out after local anesthesia with lignocaine (Xylovet; Sanofi-Synthelabo) (18).
Flow cytometric analyses.
The following monoclonal antibody clones were used: CD3-FITC (FN18; BioSource International), CD4-PE (Leu3; BD), CD8-PE (Leu2a; BD), CD16-PE (3G8; BD Biosciences — Pharmingen), CD25-PE (1HT44H3; Immunotech/Beckman Coulter Inc.), CD28-FITC (L293; BD), and HLA-DR-FITC (L243; BD), as well as isotype controls matching the IgG subclass. AGMs often demonstrate a high frequency of CD4+ T cells coexpressing CD8 (6, 49). Double-positive CD4+/CD8+ T cells express the chain at low density and not the ? chain, whereas the majority of CD4–/CD8bright cells coexpress the ? chain (49). CD8bright/CD4– cells were defined as CD8+ T cells and the CD4+/CD8low or CD4+/CD8– T cells as CD4+ T cells. Absolute numbers of lymphocyte subsets in blood were calculated using routine diagnostic counts.
RNA extraction from tissues.
PBMCs, LN lymphocytes, and BALCs were lysed in RLT-RNA lysis buffer (QIAGEN) and then were run over a QIAshredder column (QIAGEN) to ensure homogeneous lysis. Thereafter, we extracted total RNA using the RNeasy RNA extraction Mini Kit (QIAGEN) and by adding an RNAse-free DNAseI (QIAGEN) treatment on the column.
Quantification of viral RNA copy numbers in plasma by real-time PCR.
The RNA copy numbers of SIVagm.sab and SIVmac were quantified by real-time PCR. The RT-PCR assay specific for SIVagm.sab has been described elsewhere (9). Quantification of SIVmac RNA in real time was carried out using a protocol initially described by Hofmann-Lehmann et al. (15). The standard RNA was obtained from the pGEM-5Zf+ GAG plasmid (provided by A.-M. Aubertin, Universite Pasteur, Strasbourg, France).
Quantification of cellular gene expression by real-time PCR.
Total RNA primed with random hexamers was submitted to an RT step and carried out with the TaqMan Reverse Transcription Reagents kit (Applied Biosystems). The primers and TaqMan probes used for the quantification are summarized in Table 1. For the quantitation of FoxP3 and TGF-?1 gene expression, we used commercially available kits (TaqMan Assay-on-Demand; Applied Biosystems).
To normalize for RNA input, the sample content of 18SrRNA was quantified with the TaqMan assay reagents for human 18SrRNA (Applied Biosystems). We determined the expression of a given gene using the comparative (??CT) method (User Bulletin 2, ABI PRISM 7700 Sequence Detection System; http://docs.appliedbiosystems.com/pebiodocs/04303859.pdf). The expression levels of each gene normalized for 18SrRNA are expressed as n-fold difference relative to the calibrator corresponding to total cellular RNA from an unrelated sample.
Quantification of cytokines in plasma.
We measured levels of TNF-, IL-10, and TGF-?1 in plasma using the following ELISA kits: Cytoscreen monkey TNF- (Biosource International), IL-10 monkey (U-CyTech Biosciences), and TGF-?1 Multispecies Cytoscreen (Biosource International), respectively. Detection limits were 15, 2.5, and 15.6 pg/ml for TNF-, IL-10, and TGF-?1, respectively. To verify that the anti-macaque TNF- and IL-10 antibodies cross-reacted with AGM cytokines, AGM PBMCs were stimulated in vitro, and cytokines were measured in the supernatants (data not shown).
Statistical analyses.
We performed all statistical analyses using Statview (Abacus Concept). P < 0.05 was considered statistically significant. We used the Wilcoxon signed rank test to evaluate whether there was a statistically significant difference in the level of one given marker at a given time point following inoculation when compared to the preinoculation baseline. Preinoculation baselines consisted of 4 preinfection values per animal for the gene expression analysis and 7 total preinfection values for the study of plasma cytokines and of CD25+ T cell frequency and counts. The Spearman rank test was used to assess the correlation between 2 continuous variables. All analyzed markers were previously normalized in log10. We performed MANCOVA to study the simultaneous effect of the nominal factor before infection versus after SIVagm infection and (a) TGF-?1 gene expression as a covariate, on TNF-, IFN-, and IL-10 gene expressions and (b) IL-10 gene expression as a covariate, on TNF-, IFN-, and TGF-?1 gene expressions. We used the Mann-Whitney U test to compare the cytokine concentrations in plasma between SIVagm and SIVmac infections.
Acknowledgments
We thank L. An for excellent technical help. We are indebted to J.M. McCune, L. Rogge, and D. Scott-Algara for critical reading of this manuscript. C. Kornfeld received a fellowship from SIDACTION; M.J.-Y. Ploquin, a scholarship from Le Minist3re de l’Education Nationale, de la Recherche et de la Technologie; and L. Mortara and J.-F. Desoutter, fellowships from the French Agency for AIDS Research (ANRS). The study was supported by grants from the ANRS.
Footnotes
C. Kornfeld and M.J.-Y. Ploquin contributed equally to this work.
I. Pandrea and C. Apetrei’s present address is: Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana, USA.
V. Poaty-Mavoungou’s present address is: Institute for Tropical Medicine, Department of Parasitology, University of Tübingen, Tübingen, Germany.
L. Mortara’s present address is: Department of Clinical and Biological Sciences, School of Medicine, University of Insubria, Varese, Italy.
Nonstandard abbreviations used: AGM, African green monkey; BAL, bronchoalveolar lavage; BALC, BAL cell; LNLC, LN lymphocyte; SIVagm, SIV infection in African green monkeys; SM, sooty mangabey; VL, viral load.
Conflict of interest: The authors have declared that no conflict of interest exists.
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2Centre International de Recherche Medicale, Franceville, Gabon.
3Laboratoire de Retrovirologie, Institut Pasteur, Dakar, Senegal.
4 Unite de Recherche et d’Expertise Physiopathologie des Infections Lentivirales, Institut Pasteur, Paris, France.
5INSERM 567, UMR CNRS 8104, IFR 116, Departement d’Immunologie, Institut Cochin, Paris, France.
6 Universite Paris VII Denis Diderot, Paris, France.
7Commissariat a l’Energie Atomique, Fontenay aux Roses, France.
Abstract
T cell activation levels in HIV infection are predictive of AIDS progression. We searched for the immunological correlates of protection against disease progression by studying the early stages of nonpathogenic SIV infection in African green monkeys (SIVagm). The African green monkeys (AGMs) displayed high peak viremias and a transient decline in levels of blood CD4+ and CD8+ T cells between days 5 and 17 after infection. A concomitant increase in levels of CD4+DR+, CD8+DR+, and CD8+CD28– cells was detected. After the third week, T cell activation returned to baseline levels, which suggested a protective downregulation of T cell activation. A very early (24 hours after infection) and strong induction of TGF-?1 and FoxP3 expression was detected and correlated with increases in levels of CD4+CD25+ and CD8+CD25+ T cells. This was followed by a significant increase in levels of IL-10, whereas IFN- gene upregulation was more transient, and levels of TNF- and MIP-1/? transcripts did not increase in either blood or tissues. The profiles were significantly different during primary SIV infection in macaques (SIVmac); that is, there was a delayed increase in IL-10 levels accompanied by moderate and persistent increases in TGF-? levels. Together, our data show that SIVagm infection is associated with an immediate antiinflammatory environment and suggest that TGF-? may participate in the generation of Tregs, which may prevent an aberrant chronic T cell hyperactivation.
Introduction
HIV-1 infection is characterized by chronic T cell hyperactivation. Increased T cell activation from the initial stage of infection has predictive value for AIDS progression even before seroconversion and independently of viral load (VL) (1-4). The precise mechanisms leading to this aberrant chronic T cell activation remain unclear. The study of SIV infections in nonhuman primate models has contributed to an understanding of the mechanisms underlying CD4+ T cell depletion and AIDS. The animal model that best reflects HIV infection in humans is SIV infection in macaques (SIVmac). SIV infections are generally only nonpathogenic in African nonhuman primate species, the natural hosts of SIV, such as African green monkeys (AGMs), mandrills (MNDs), sooty mangabeys (SMs), and chimpanzees (CPZs) (5). During SIVagm, SIVsm, SIVmnd-1, and SIVcpz infections in their respective hosts, VLs have been shown to be similar to those recorded for pathogenic HIV-1 and SIVmac infections (6). Furthermore, SIVagm replicates in lymphoid tissues, including the gut (6, 7). Thus, these studies have shown that viral replication alone is not sufficient to drive CD4+ T cell decline.
Despite high levels of VLs, natural hosts show distinct lymphocyte activation profiles during chronic infection compared with humans and macaques. Naturally infected AGMs and SMs do not exhibit significant LN follicular hyperplasia (8, 9) or infiltration of CD8+ cells into LN germinal centers (9). Naturally infected MNDs and SMs exhibit nearly unchanged percentages of CD4+DR+ T cells and only slight increase in the percentages of CD8+DR+ T cells in blood (10, 11). Long-term SIVsm infection in SMs is characterized by normal T cell turnover and decreased IFN- expression that correlates with low CTL activity (8, 11, 12). Finally, chronically infected AGMs, SMs, and CPZs failed to show enhanced peripheral CD4+ T cell apoptosis levels (11, 13). Data on T cell activation and cytokine profiles during the initial stage of SIVagm in AGMs are crucially needed in order to understand the very early events that will shape immune responses and prevent the aberrant chronic T cell activation despite continuous viral replication and the following questions must be addressed: (a) Are the T cells activated during primary infection in response to SIVagm? (b) Are the cytokine profiles pro- or antiinflammatory? (c) Is the low activation associated with tolerance toward SIVagm?
To address these questions, we studied T cell activation patterns in distinct tissues of SIVagm-infected AGMs during primary infection. CD4+ and CD8+ T cell subpopulations were examined in peripheral blood as well as in secondary lymphoid organs. To analyze the early orientation of the immune response, we compared the cytokine profiles of 3 distinct compartments — blood (i.e., at a systemic level), LN, and peripheral tissue (by bronchoalveolar lavage [BAL] — at initiation and effector sites of the immune response. This study reveals that SIVagm infection in AGMs can lead to a transient T cell activation during primary infection but that AGMs mount an immediate antiinflammatory cytokine environment. This raises the possibility of a Treg induction, which may then control T cell turnover rates.
Results
Transient decline in blood CD4+ T cells during primary SIVagm infection.
Nine AGMs infected with SIVagm.sab92018 as well as 2 control animals were included in this study. To allow comparison between pathogenic SIVmac and nonpathogenic SIVagm infection under the same experimental conditions, we included 2 SIVmac251-infected macaques as well as 2 control macaques as internal control for phenotypic analysis of CD4+ and CD8+ T cells (Figures 1). Both infected macaques progressed rapidly toward disease, developing AIDS within 7 months after infection. Plasma VLs were very similar in SIVmac-infected macaques and SIVagm-infected AGMs (Figure 1). VLs peaked between 6 and 10 days after infection (3 x 107 to 8 x 107 copies/ml of plasma). After day 35, the plasma VLs ranged from of 2 x 103 to 8 x 104 copies/ml and were within the range previously recorded by us and others in chronically infected AGMs and macaques (6, 14, 15).
We then compared the early T cell dynamics in macaques and AGMs. In both SIVmac-infected macaques (MACQ172 and MACQ205), the blood CD4+/CD8+ T cell ratios decreased starting from day 10 after infection. (Figure 2A). After day 10, the absolute number CD4+ T cells remained low compared with baseline, whereas numbers of CD8+ T cells returned to baseline (data not shown). In AGMs, an early significant decline was also detected, reaching a maximum between days 7 (P < 0.0001) and 10 (P < 0.0001) (Figure 2B). This decline was due to a more pronounced decrease in the absolute number of CD4+ T cells compared with CD8+ T cells (data not shown). Both CD4+ and CD8+ T cell counts reached preinfection values after day 10 and remained stable. There was a negative correlation between plasma SIVagm RNA levels and CD4+ T cell counts ( = –0.541; P < 0.0001) during primary SIVagm infection. Together, our results indicate that primary SIVagm infection in AGMs, like pathogenic SIVmac infections in macaques, can lead to a significant decrease in the absolute number of CD4+ T cells in blood.
To determine whether modulations in blood T cell subsets are reflected in secondary lymphoid tissues, we analyzed CD4+ and CD8+ T cell percentages in LN during SIVagm and SIVmac infections. Whereas the 2 mock-infected control macaques had a stable CD4+/CD8+ T cell ratio in LN throughout the experimental follow-up, both SIVmac-infected macaques exhibited a decrease during the acute phase of the infection. This decline was more pronounced during the chronic phase of infection (Figure 2C). By contrast, SIVagm-infected AGMs showed no significant changes in CD4+/CD8+ T cell ratios within LN at any time during the period of observation (Figure 2D). This observation is consistent with our previous finding of a lack of CD8+ T cell infiltrations into LN germinal centers (9).
Transient increases of activated CD4+ and CD8+ T cells in blood during primary SIVagm infection.
To determine whether decreases in circulating CD4+ and CD8+ T cells in AGMs were associated with changes in T cell activation levels, we performed a comparative analysis of T cell activation markers in blood and LN cells during primary SIVagm and SIVmac infection. We focused our comparative analysis on 2 cell-surface markers (DR and CD28), whose expression levels correlate with disease progression in HIV-1/SIVmac infections (1, 3, 16).
We analyzed T cell activation in macaques and AGMs. None of the SIVmac-infected macaques studied showed significant changes in the proportion of circulating DR-expressing CD4+ T cells during primary infection, but the proportion was enhanced after day 28 (Figure 3A). By contrast, in AGMs, there was a significant increase in the percentages of CD4+DR+ cells only at early time points, i.e. at days 7 (P = 0.003) and 10 (P < 0.0001) (Figure 3B). Activation profiles within the circulating CD8+ T cell population mirrored those observed for circulating CD4+ T cells. During primary SIVmac infection in macaques, an early, slight increase in CD8+DR+ T cells was followed by a persistent increase during chronic infection (Figure 3C) concomitantly to a continuous decrease of CD8+CD28+ T cells (data not shown). In AGMs, the baseline percentage of DR+ peripheral blood CD8+ T cells was more variable than that seen in macaques (ranging from 8% to 62%). After SIVagm infection, the percentages of CD8+DR+ T cells were significantly increased between days 8 and 21 (P 0.044 for each) (Figure 3D). Values then declined to baseline levels by day 28. AGMs also exhibited significant decreases in CD8+CD28+ T cells between days 5 and 21 (P 0.035 for each) (data not shown). After day 21, the percentages of CD8+CD28+ T cells returned to their preinfection values. We thus confirm a lack of increased T cell activation during the chronic phase of infection and reveal for the first time to our knowledge that T cell activation can occur during primary SIVagm infection.
The percentages of activated CD8+ T cells in AGM LNs remain unaltered after SIVagm infection.
There was no significant change in the frequency of LN CD4+DR+ and CD4+CD28+ T cells from macaques and AGMs after infection (data not shown). An increase in CD8+DR+ and CD8+CD28– T cells was observed after 60 days in the SIVmac-infected macaques, whereas in AGMs, no significant changes were evidenced in LN (data not shown). During the chronic phase of infection, there is therefore, as expected, a lower level of CD8+ T cell activation in the LN of AGMs compared with macaques. That the frequencies of DR- and CD28-positive CD8+ T cells in LN were unaltered during primary infection contrasts with the changes detected in the periphery but is consistent with the absence of CD4+/CD8+ T cell ratio changes.
Expression of RANTES, CCR1, and CCR5 transcripts is transiently upregulated in acutely SIVagm-infected AGMs.
The presence of higher levels of activated T cells and of infiltrating cells in LN in pathogenic infections may result from a heightened state of inflammation. We searched for differences in the early pro- and antiinflammatory cytokine profiles induced during primary infection. We analyzed the profiles in AGMs in 3 distinct compartments: PBMCs, LN lymphocytes (LNLCs), and BAL cells (BALCs), a peripheral compartment that has been found to present signs of inflammation in acutely SIVmac-infected macaques (17, 18). The 3 compartments were studied in 5 AGMs (3 randomly selected SIVagm-infected AGMs and the 2 mock-infected control AGMs). We first measured ? chemokine expression as a marker for inflammation.
During primary SIVagm infection, RANTES gene expression was significantly increased (at days 7, 10, 17, 21, and 30 in PBMCs (P 0.04 for each) and in BALCs from days 7 to 30 (P 0.027 for each). In LN, increases in RANTES expression levels were observed in both SIV- and mock-infected animals and were thus not considered to be specifically induced by SIVagm infection (Figure 4, A and B). Changes in the expression levels of MIP-1 and MIP-1? genes were less frequently observed in infected animals than for RANTES and/or also observed in the mock-infected AGMs (Figure 4, A and B) after infection and throughout acute infection (P 0.027 for each). In summary, these observations demonstrate that SIVagm infection in AGMs leads to induction of RANTES, CCR1, and CCR5 gene expression in PBMCs and BALCs, whereas no infection-specific increases were detected in LN. Furthermore, no or only very modest gene expression changes of MIP-1 and MIP-1? were detected.
Increased IL-10/TNF- ratios during primary SIVagm infection.
To further explore the possibility that proinflammatory cytokine expression is dampened during SIVagm infection, we analyzed the gene expression levels of TNF- and IL-10. Their expression was first measured in the 3 tissue compartments of the same 5 AGMs analyzed for chemokine gene expression (Figure 5), as well as in the 6 other AGMs at earlier time points with shorter intervals in PBMCs (Figure 6). TNF- gene expression was increased in PBMCs at days 5, 10, and 17 after infection (P < 0.042 for each) and in LN at days 5 (P = 0.008) and 21 (P = 0.007), but mock-infected control AGMs also exhibited similar TNF- gene expression changes in both PBMCs and LN (Figure 5). The increases in TNF- expression therefore appear not to be SIV specific but rather due to other factors, such as repeated sampling and/or injection of plasma. In BALCs of both SIVagm- and mock-infected AGMs, no significant changes in TNF- gene expression were detected at any time during the study period (Figure 5B), in contrast to what has been observed in macaques (19). The expression of the IL-10 gene in PBMCs and BALCs from SIVagm-infected AGMs (Figure 5A), but not mock-infected controls (Figure 5B), was significantly increased starting from day 7 until day 30 after infection (P < 0.05 for each). In LN, IL-10 gene expression was significantly increased at days 14 (P = 0.043) day 21 (P = 0.008) in infected AGMs and significantly increased at day 14 (P = 0.043) in mock-infected animals.
In summary, the above studies indicated TNF-/IL-10 ratios in favor of IL-10, specially in PBMCs and BALCs. We extended our analysis of TNF- and IL-10 gene expression in PBMCs to the 6 other AGMs. The kinetics of sampling were similar to those carried out above, except that we also collected blood at earlier time points (days 1 and 3 after infection), since the inflammatory balance might be established very early. The 6 AGMs also exhibited the same TNF-/IL-10 ratio in favor of IL-10 in PMBC (Figure 6A).
To further confirm this antiinflammatory cytokine environment in response to SIVagm infection, we measured TNF- and IL-10 protein concentrations in plasma. Plasma TNF- in all 6 SIVagm-infected AGMs measured throughout the acute phase and at day 120 always remained under the detection limit of the assay (15 pg/ml) (data not shown). In contrast, we detected heightened levels of IL-10 in plasma during primary infection, and the increases were statistically significant at days 6, 8, 10, and 13 (P 0.002 for each) (Figure 6B). In order to confirm that the early antiinflammatory cytokine environment was specific for SIVagm infection, we assayed IL-10 protein concentrations in 6 SIVmac251-infected rhesus macaques. They were significantly decreased from days 1 to 10 (P 0.019 for each) and significantly increased only later on day 21 (P = 0.024) (Figure 6B). These data support the previous conclusion that an antiinflammatory environment is induced early in SIVagm infection compared with pathogenic SIVmac infection.
Transient IFN- gene expression increases during primary SIVagm infection.
IL-10 has been implicated in the suppression of Th1-type immune responses. We therefore followed the changes in the gene expression of a typical Th1-type cytokine, IFN-. We first compared IFN- gene expression in the 3 tissue compartments of the 3 SIVagm-infected and 2 mock-infected AGMs. In LNLCs and BALCs, no statistically significant changes in IFN- gene expression were detected at any time after SIVagm infection (Figure 5). In PBMCs, the SIVagm-infected animals demonstrated a significant increase at days 5 (P = 0.018) and 10 (P = 0.033). In the 6 other AGMs, we also observed a similar transient increase in PBMCs at days 10 (P = 0.005) and 16 (P = 0.019) (Figure 6A). To summarize, the analysis of IFN- gene expression detected increases only in PBMCs, but not in LNLCs and BALCs, in contrast to IL-10. In PBMCs, the increases in IFN- gene expression appeared to be more transient than those in IL-10 expression.
Immediate increases of CD4+CD25+ and CD8+CD25+ T cells in blood.
The above data reveal that primary SIVagm infection in AGMs is associated with a transient activation of T cells, TNF-/IL-10 ratios in favor of IL-10, and a weak IFN- response. These observations raised the hypothesis that Tregs are induced after SIVagm infection of AGMs. To evaluate this possibility more closely, we analyzed the frequency of CD4+CD25+ and CD8+CD25+ T cells in 6 AGMs from time points directly after infection (day 1) through day 430 after infection.
At days 1 and 6, percentages of CD4+CD25+ and CD8+CD25+ T cells in peripheral lymphocytes were significantly increased (P 0.014 for each) (Figure 7, A and B). At days 13, 16, and 21, percentages of CD8+CD25+ T cells rebounded (P 0.038 for each) after a decrease at days 8 and 10 (P 0.0005 for each), and throughout the chronic phase, the percentages of CD4+CD25+ and CD8+CD25+ T cells were significantly lower (P 0.026 for each) relative to preinfection levels (Figure 7, A and B). A similar pattern was observed for the absolute counts of CD4+CD25+ and CD8+CD25+ T cells, the percentages of CD4+CD25+ in CD4+ T cells, and the percentages of CD8+CD25+ in CD8+ T cells (data not shown).
Immediate increases of TGF-? 1 and FoxP3 gene expressions during SIVagm.
CD25 is not a specific marker of Tregs, as it is also upregulated upon conventional T cell activation. Recently, a transcription factor (FoxP3) has been described to be specifically expressed in humans by thymic and peripheral regulatory CD8+ and CD4+CD25+ T cells (20, 21). To determine whether the increased levels of CD4+CD25+ and CD8+CD25+ T cells observed in SIVagm-infected AGM were Tregs or conventional activated T cells, we quantified FoxP3 transcript levels in PBMCs of the same 6 animals. We found significantly increased FoxP3 levels at days 1 and 6 after infection (Figure 7C). Correlations between both numbers and percentages of CD4+CD25+ T cells and FoxP3 gene expression trended toward significance ( = 0.280, P = 0.076 and = 0.278, P = 0.079, respectively). A statistically significant positive correlation was observed between FoxP3 gene expression and both the numbers ( = 0.548, P = 0.0003) and percentages of CD8+CD25+ T cells ( = 0.554, P = 0.0002).
It has been previously found that FoxP3 gene expression and subsequent conversion of naive CD4+CD25– T cells into CD4+CD25+ Tregs is induced by TGF-?1 (22). Accordingly, we monitored TGF-?1 gene expression in PBMCs during SIVagm infection. We observed statistically significant increased levels of TGF-?1 (Figure 7D). The changes were observed very early at days 1, 3, and 13–16 after infection (P = 0.0008, P = 0.004, and P = 0.028, respectively) and there was a trend toward significance at day 6 (P = 0.079). The levels of FoxP3 and TGF-?1 gene expression were highly correlated ( = 0.863, P < 0.0001). Interestingly, immediate increases (day 1 after infection) were only observed for FoxP3 and TGF-?1, whereas the expression of other cytokines, such as IL-10, was only increased several days later during primary infection. According to covariance analysis, TGF-?1 gene expression had a significant effect after SIVagm infection on gene expressions of the other cytokines (P = 0.044, multivariate analysis of covariance [MANCOVA]), whereas IL-10 expression did not have a significant effect (P = 0.72, MANCOVA). TGF-?1 gene expression did not correlate with the numbers of CD4+CD25+ T cells ( = 0.251, P = 0.112) but did correlate with the percentages of CD4+CD25+ T cells ( = 0.372, P = 0.019) and, as had FoxP3, with both numbers ( = 0.557, P = 0.0002) and percentages of CD8+CD25+ T cells ( = 0.554, P = 0.0002).
To confirm the increases of TGF-?1 gene expression at the protein level, we assayed TGF-?1 in plasma during primary SIVagm infection. Our results clearly revealed a statistically significant increase in plasma TGF-?1 at day 1 after infection (P < 0.0001) (Figure 7E). Then plasma concentrations returned to baseline levels at days 3 and 6 (except for 2 of the 6 animals at day 3 [SAB97008 and SAB98013 and at day 6 [SAB01015 and SAB00020, for which concentrations of plasma TGF-?1 remained higher [2- to 3-fold] compared with their preinfection levels). Throughout days 10–21, concentrations of plasma TGF-?1 were lower than preinfection baseline levels (P < 0.0001). Again, we found that TGF-?1 concentrations in plasma were significantly correlated with the absolute numbers of CD8+CD25+ T cells in blood ( = 0.375, P = 0.033). This early rise of TGF-?1 concentrations in plasma is consistent with our previous conclusion that an antiinflammatory cytokine environment is immediately induced after SIVagm infection.
In order to study whether or not the early TGF-?1 increase is present during primary pathogenic SIVmac infection, we assayed TGF-?1 in plasma from the 6 SIVmac251-infected rhesus macaques for which we had assayed IL-10 concentrations in plasma. We observed significant increases at days 1, 3, 6, 10, 13, and 16 (P 0.043 for each) (Figure 7F). Nevertheless, the increases at day 1 after SIVmac infection were significantly lower compared with those after SIVagm (P = 0.037, Mann-Whitney U test). Interestingly, the profiles were inversed after VL peaked (days 10–21). Indeed, TGF-?1 concentrations in infected macaques were significantly higher than those in infected AGMs at days 10 and 16 (P = 0.037 and P = 0.025, respectively, Mann-Whitney U test). Therefore, the patterns were different in pathogenic and nonpathogenic infections, with an immediate strong increase in TGF-?1 levels after primary SIVagm infection and a moderate persistent increase after primary SIVmac infection.
Discussion
The present study on nonpathogenic SIVagm infection adds significantly to our understanding of critical factors involved in AIDS protection. Several mechanisms have been proposed as driving progression toward AIDS in humans and macaques: (a) accelerated cell death, (b) impaired T cell generation, and (c) accelerated turnover leading to exhaustion. Our results show that during primary SIVagm infection in its natural host, a decline in peripheral CD4+ T cells to levels similar to those found in pathogenic SIVmac can occur. The CD4+ T cell decline was slightly greater than that of CD8+ T cells and may thus partially result from virus-induced cell death. SIVagm has indeed been shown to be as cytopathic as HIV-1 or SIVmac in vitro (23). However, since CD8+ T cells also decrease, albeit to a lesser extent than CD4+ T cells, an additional reason for loss of T cells in the periphery could be bystander cell death and/or migration to LN. Bystander cell death of CD8+ T cells has been demonstrated in several natural hosts, including AGMs (13). Although we did not see any changes in the CD4+/CD8+ T cell ratio in LN, a slight, equal increase in both CD4+ and CD8+ T cells cannot be excluded. If such an increase did occur, it must be moderate in comparison with that in SIVmac-infected macaques, since no lymphadenopathy has been evidenced in LN of SIVagm-infected AGMs (9). The detection of increased percentages of activated CD8+ T cells during primary infection in blood but not in LN of AGMs might be explained by a release of activated cells in the circulation in AGMs. In contrast, the higher and more persistent levels of activated T cells in LN in macaques suggests a sequestration of activated cells in LN during pathogenic HIV-1/SIVmac infections. This hypothesis is consistent with the lack of infiltration of CD8+ cells in germinal centers of LN of infected AGMs (9).
The lack of chronic hyperactivation seems indeed to be a common feature of nonpathogenic SIV infections in natural hosts (8-11, 13, 24). In HIV infection, evidence has been accumulating to support the concept that the degree of activation is the best predictor of disease progression, whereas VL is only indirectly associated with progression (1, 2, 4). CD8+ T cell activation at early stages in HIV infection is determinant of the rate at which CD4+ T cells are lost over time (3). Thus, it is critical to identify the mechanism(s) that protect against the harmful hyperactivation of the immune system. It has been proposed that protection in AGMs could be due to tolerance against a SIVagm protein (25). Our study reveals a state of increased T cell activation in peripheral blood subsequent to SIVagm infection, which thus clearly demonstrates that AGMs respond to SIVagm infection and that protection is not due to a central tolerance mechanism. However, our data on IFN- gene expression suggest a low Th1 response. We found no increase in LNLCs and BALCs and increases in PBMCs only between days 5 and 16 after infection. This is consistent with the decreased IFN- production in CD4+ T cells and low CTL frequencies reported in chronically SIVsm-infected SMs (11, 12). It is beneficial to maintain a Th1-type cytokine secretion pattern when it is associated with efficient CTL responses during chronic HIV-1 infection, as observed in some LTNPs (26). Although, Th1 responses are protective, they may be harmful if they are associated with strong, generalized immune activation (12, 27). An et al. indeed reported that a TNF-–inducible promoter variant of IFN- (–179T) accelerates loss of CD4+ T cells in humans (28). In clinical trials with IFN-, both acceleration of AIDS progression and decline of CD4 cell counts have been reported (29). Recently, it has been shown that the long-term challenge outcome in vaccinated macaques was correlated with the relative balance between SIV-specific IFN- T cell responses and nonspecific IFN-–driven inflammation, the latter being seen in animals in which viral replication cannot be controlled (30).
We found that an antiinflammatory environment is established early during SIVagm infection. Our data on ? chemokine expression are in contrast to those on HIV-1 infection in humans or SIVmac-infected macaques, where significant increases of RANTES, MIP-1, MIP-1?, and CCR5 gene expressions have been observed in PBMCs, LN, and BALCs (17, 18). Recently, it has been reported that high ? chemokine expression levels in lymphoid tissues of SIVmac-infected macaques contribute to nonspecific inflammation and immune activation as well as to high VL in lymphoid tissues (31). RANTES is chemotactic mainly for monocytes and CD4+ memory T cells, whereas MIP-1 and MIP-1? have been shown to attract CD8+ T cells (32). Therefore, the lack of MIP-1 and MIP-1? gene overexpression in SIVagm infection might be associated with the lack of CD8+ lymphocytosis and CD8+ infiltration in LN germinal centers (9).
SIVagm-infected AGMs also exhibited no signs of significant TNF- increases. This contrasts with TNF- increases detected in acutely SIVmac-infected macaques and in HIV-infected humans (14, 33, 34). We previously showed, for instance, that SIVmac-infected cynomolgus macaques produce up to 1,500 pg TNF-/ml of serum during primary infection (35). The lower TNF- expression during SIVagm infection was not due to a constitutive impairment, since we detected TNF- (up to 100 pg/ml) after ConA stimulation of AGM mononuclear cells (data not shown). Rather, as shown here, AGMs exhibit a significant increase of IL-10 during primary infection. Although increased IL-10 expression levels have been associated with disease progression during later stages of HIV-1 infection (36), a higher stimulus-induced IL-10 mRNA expression, together with a lower constitutive TNF- mRNA expression — similar to what we observed in AGMs — were indeed associated with slower disease progression in HIV-1-infected children (37). Moreover, a mutation in the IL-10 promoter region, resulting in constitutively reduced IL-10 gene expression, is associated with a more rapid progression toward AIDS in HIV-1–infected patients (38). One explanation for the discrepancy in the findings on the role of IL-10 might thus be related to the different disease stages studied. Here our data show that during acute SIVmac251 infection in rhesus macaques, IL-10 secretion in plasma was indeed significantly increased, but this occurred later than in nonpathogenic SIVagm infection. Previous studies on SIVmac-infected macaques showed enhanced levels of IL-10 gene expression in PBMCs in a few animals during primary SIVmac infection, but this expression was generally accompanied by strong TNF- gene expressions (14, 19). Together, our data reveal early induction of an antiinflammatory environment in response to SIVagm infection in AGMs. This may be what determines the subsequent low, generalized immune activation levels.
This antiinflammatory environment might be induced in AGMs by TGF-?1, whose expression is upregulated as early as 24 hours after infection to levels significantly higher than those in macaques, which show greater levels only starting at day 10. Omer et al. observed that in Plasmodium yoelii–infected mice, a burst of TGF-?1 within 24 hours inhibits IFN- and TNF- responses, whereas increases of TGF-?1 4 days later do not (39). Moreover, the environment in which TGF-?1 is produced starting from the second week after infection is also different between pathogenic and nonpathogenic SIV infections, since in macaques it is concomitant with a proinflammatory cytokine balance (14, 17-19, 33, 35). The response by TGF-?1 that is weaker early and stronger thereafter in macaques may reflect an inability to prevent a fatal overwhelmed immune response and a continuous need for tissue repair mechanisms, as conferred by TGF-?1 (40), due to an inability to resorb virus-driven inflammation. In Plasmodium yoelii–infected mice, the major producers of TGF-?1 are adherent cells, CD8+ and CD25+ cells (39). In AGMs, we found that the most statistically significant correlation of TGF-?1 (transcript and plasma levels) was with CD8+CD25+ T cells. It remains to be determined which cells express TGF-?1 during primary nonpathogenic SIVagm infection and pathogenic SIVmac infection, but CD8+CD25+ T cells could be one of the major sources of TGF-?1 during primary SIVagm infection.
TGF-?1 might be responsible for the induction of FoxP3, which correlates with the increases of CD25+ cells, in particular CD8+CD25+ T cells. Although the presence of Tregs in AGMs needs to be confirmed by functional studies, the hypothesis that they are induced is strongly supported by our findings. So far, it is unclear whether they would correspond to natural (Trn) or to peripherally induced (Tr1, TH3) Tregs or both. One cannot exclude the possibility that a first expansion of FoxP3+ Trn might enhance the induction of peripheral IL-10–producing FoxP3– Tregs, eventually associated with the repeated Ag stimulations in the absence of proinflammatory signals (41). Further studies are needed to determine to which subset of CD8+ and/or CD4+ Tregs the cells might belong.
Such an induction of Tregs could explain the downregulation of T cell activation in AGMs. The presence of CD4+ and CD8+ Tregs has been described ex vivo during the chronic phase in HIV-1–infected patients (42-46). Some of these studies proposed that an impaired generation of CD4+CD25+ Tregs might be at the origin of the uncontrolled T cell hyperactivation (44, 45), whereas others proposed that Tregs could be at the origin of immunosuppression in HIV-1 infection (42, 46). Thus, it is not clear so far whether they play a protective role against T cell hyperactivation or rather contribute to immunosuppression in HIV infection. Our data are in favor of a protective role of Tregs. We and others have previously shown that primary SIVmac infection is associated with increases in CD4+CD25+ T cells in blood between days 7 and 21 after infection but with unchanged CD8+CD25+ T cell levels (47, 48). One can hypothesize that SIVmac-infected macaques exhibit chronic abnormal T cell activation due to a delayed expansion and/or a lack of CD8+ Treg induction.
In summary, our study describes, for the first time to our knowledge, the balance between the expression of pro- versus antiinflammatory cytokines within the first days after infection during nonprogressive SIV infection, and suggests a mechanism for avoiding harmful generalized immune activation after lentiviral infection. Our data reveal an immediate antiinflammatory balance in AGMs in response to SIVagm infection. This balance might be primed by TGF-?1, which is induced early and strongly and which might lead to the very early increase (starting from day 1 after infection) of FoxP3 expression. The potential rapidly induced CD4+ and CD8+ Tregs might temper the proinflammatory cytokine cascade and protect against chronic hyperactivation. These new insights into the early cytokine balance and potential protective role of Tregs are crucial for the research on novel immune intervention strategies for the prevention and treatment of AIDS.
Methods
Viral stocks.
The generation and titration of the SIVagm.sab92018 virus stock has been described elsewhere (9). The cell-free SIVmac251 stock was kindly provided by A.M. Aubertin (Universite Louis Pasteur, Strasbourg, France). We used AGMs inoculated with SIV-negative plasma as mock-infected controls. This plasma was collected from the same monkey from which samples were used to prepare the SIVagm stock. The negative control plasma for macaques was obtained from an unrelated donor macaque.
Animals and infections.
AGMs used in these studies belonging to the Chlorocebus sabaeus species were housed at the Pasteur Institute facility in Dakar, Senegal. Cynomolgus macaques (MAC141A, MACN894, MACQ172, and MACQ205) were housed at the Centre International de Recherche Medicale in Franceville, Gabon. Chinese rhesus macaques (MAC1058, MAC1073, MAC1076, MAC1085, MAC1094, and MAC1099) were housed at the Pasteur Institute facility in Ile-de-France, France. All animals were housed according to national guidelines and institutional policies. Animal care and use were approved by the Committee for Ethics and Animal Experimentation at the International School of Science and Veterinary Medicine in Dakar, Senegal. For infections, 100 and 300 TCID50 (where TCID50 is the tissue culture infective dose that affects 50% of inoculated cultures) SIVagm.sab92018 were intravenously inoculated into 3 (SAB98007 SAB98008 and SAB98011 and 6 (SAB97008 SAB98013 SAB00020 SAB01013 SAB01015and SAB02003 sabaeus monkeys (a sub-species of AGMs), respectively. The 3 cynomolgus macaques were intravenously inoculated with 100 TCID50 of SIVmac251. The 6 rhesus macaques were intravenously inoculated with 10 AID50 (where AID50 is the animal infective dose that affects 50% of inoculated cultures) of SIVmac251. Four control animals (2 sabaeus and 2 cynomolgus macaques) were intravenously inoculated with the corresponding volume (0.03–1 ml) of species-specific SIV-negative plasma. Although, we cannot exclude the possibility that differences between SIVagm.sab92018 and SIVmac251 infections described here are virus dependent, we used models in which monkeys were infected with their species-specific SIVs.
Specimen collection.
For every monkey included in the protocol, 5 ml of whole blood was collected in EDTA K2 tubes. PBMCs were isolated by Ficoll-Hypaque (Sigma-Aldrich) gradient centrifugation, after which plasma was recovered and clarified from cellular debris and residual platelets by additional centrifugation at 3,000 g for 5 minutes.
Excisional axillar and inguinal LN biopsies were performed, and tissues were mechanically dissociated. LNLCs were separated from the remaining tissue by passage through a 100-mm nylon mesh. A total of 2 x 106 fresh cells were used for flow cytometry.
BAL was carried out after local anesthesia with lignocaine (Xylovet; Sanofi-Synthelabo) (18).
Flow cytometric analyses.
The following monoclonal antibody clones were used: CD3-FITC (FN18; BioSource International), CD4-PE (Leu3; BD), CD8-PE (Leu2a; BD), CD16-PE (3G8; BD Biosciences — Pharmingen), CD25-PE (1HT44H3; Immunotech/Beckman Coulter Inc.), CD28-FITC (L293; BD), and HLA-DR-FITC (L243; BD), as well as isotype controls matching the IgG subclass. AGMs often demonstrate a high frequency of CD4+ T cells coexpressing CD8 (6, 49). Double-positive CD4+/CD8+ T cells express the chain at low density and not the ? chain, whereas the majority of CD4–/CD8bright cells coexpress the ? chain (49). CD8bright/CD4– cells were defined as CD8+ T cells and the CD4+/CD8low or CD4+/CD8– T cells as CD4+ T cells. Absolute numbers of lymphocyte subsets in blood were calculated using routine diagnostic counts.
RNA extraction from tissues.
PBMCs, LN lymphocytes, and BALCs were lysed in RLT-RNA lysis buffer (QIAGEN) and then were run over a QIAshredder column (QIAGEN) to ensure homogeneous lysis. Thereafter, we extracted total RNA using the RNeasy RNA extraction Mini Kit (QIAGEN) and by adding an RNAse-free DNAseI (QIAGEN) treatment on the column.
Quantification of viral RNA copy numbers in plasma by real-time PCR.
The RNA copy numbers of SIVagm.sab and SIVmac were quantified by real-time PCR. The RT-PCR assay specific for SIVagm.sab has been described elsewhere (9). Quantification of SIVmac RNA in real time was carried out using a protocol initially described by Hofmann-Lehmann et al. (15). The standard RNA was obtained from the pGEM-5Zf+ GAG plasmid (provided by A.-M. Aubertin, Universite Pasteur, Strasbourg, France).
Quantification of cellular gene expression by real-time PCR.
Total RNA primed with random hexamers was submitted to an RT step and carried out with the TaqMan Reverse Transcription Reagents kit (Applied Biosystems). The primers and TaqMan probes used for the quantification are summarized in Table 1. For the quantitation of FoxP3 and TGF-?1 gene expression, we used commercially available kits (TaqMan Assay-on-Demand; Applied Biosystems).
To normalize for RNA input, the sample content of 18SrRNA was quantified with the TaqMan assay reagents for human 18SrRNA (Applied Biosystems). We determined the expression of a given gene using the comparative (??CT) method (User Bulletin 2, ABI PRISM 7700 Sequence Detection System; http://docs.appliedbiosystems.com/pebiodocs/04303859.pdf). The expression levels of each gene normalized for 18SrRNA are expressed as n-fold difference relative to the calibrator corresponding to total cellular RNA from an unrelated sample.
Quantification of cytokines in plasma.
We measured levels of TNF-, IL-10, and TGF-?1 in plasma using the following ELISA kits: Cytoscreen monkey TNF- (Biosource International), IL-10 monkey (U-CyTech Biosciences), and TGF-?1 Multispecies Cytoscreen (Biosource International), respectively. Detection limits were 15, 2.5, and 15.6 pg/ml for TNF-, IL-10, and TGF-?1, respectively. To verify that the anti-macaque TNF- and IL-10 antibodies cross-reacted with AGM cytokines, AGM PBMCs were stimulated in vitro, and cytokines were measured in the supernatants (data not shown).
Statistical analyses.
We performed all statistical analyses using Statview (Abacus Concept). P < 0.05 was considered statistically significant. We used the Wilcoxon signed rank test to evaluate whether there was a statistically significant difference in the level of one given marker at a given time point following inoculation when compared to the preinoculation baseline. Preinoculation baselines consisted of 4 preinfection values per animal for the gene expression analysis and 7 total preinfection values for the study of plasma cytokines and of CD25+ T cell frequency and counts. The Spearman rank test was used to assess the correlation between 2 continuous variables. All analyzed markers were previously normalized in log10. We performed MANCOVA to study the simultaneous effect of the nominal factor before infection versus after SIVagm infection and (a) TGF-?1 gene expression as a covariate, on TNF-, IFN-, and IL-10 gene expressions and (b) IL-10 gene expression as a covariate, on TNF-, IFN-, and TGF-?1 gene expressions. We used the Mann-Whitney U test to compare the cytokine concentrations in plasma between SIVagm and SIVmac infections.
Acknowledgments
We thank L. An for excellent technical help. We are indebted to J.M. McCune, L. Rogge, and D. Scott-Algara for critical reading of this manuscript. C. Kornfeld received a fellowship from SIDACTION; M.J.-Y. Ploquin, a scholarship from Le Minist3re de l’Education Nationale, de la Recherche et de la Technologie; and L. Mortara and J.-F. Desoutter, fellowships from the French Agency for AIDS Research (ANRS). The study was supported by grants from the ANRS.
Footnotes
C. Kornfeld and M.J.-Y. Ploquin contributed equally to this work.
I. Pandrea and C. Apetrei’s present address is: Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana, USA.
V. Poaty-Mavoungou’s present address is: Institute for Tropical Medicine, Department of Parasitology, University of Tübingen, Tübingen, Germany.
L. Mortara’s present address is: Department of Clinical and Biological Sciences, School of Medicine, University of Insubria, Varese, Italy.
Nonstandard abbreviations used: AGM, African green monkey; BAL, bronchoalveolar lavage; BALC, BAL cell; LNLC, LN lymphocyte; SIVagm, SIV infection in African green monkeys; SM, sooty mangabey; VL, viral load.
Conflict of interest: The authors have declared that no conflict of interest exists.
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