摘要: 隐孢子虫是一种属于顶端复合物门的寄生性原虫，感染此虫引起的隐孢子虫病是一种全球性的人畜共患寄生虫病。免疫力低下、功能缺陷或免疫抑制的患者感染此虫后，可引起严重胃肠炎并伴有水样腹泻，导致大量体液丢失而危及生命。艾滋病患者中感染率高达48%左右，长期的严重腹泻是艾滋病病人的重要致死因素之一。该病对人类公共卫生造成很大威胁，国外暴发流行时有报告。国内外一些学者相继利用核酸限制性片段长度多态性分析、基因克隆、分子杂交和聚合酶链式反应等分子生物学技术，对隐孢子虫核酸分子进行了大量的研究并取得了一些重要进展，并在隐孢子虫卵囊检测、隐孢子虫病的诊断、流行病学调查及虫种鉴定和虫株分型中得到广泛应用。

    关键词:隐孢子虫;聚合酶链式反应(PCR);分型;诊断

    Advancement of application of molecular biological technique in the research of cryptosporidiosis.

    ZHAI De-fu，ZHANG Dian-bo，LIU Ke-yi.

    (Shandong Provincial Institute of Parasitic Diseases，Jining272033，Shandong，P.R.China)

    Abstract:Objective Cryptosporidium，a protozoan of Apicocomplexan that invades the intestinal epithelium of a wide range of mammalian hosts，causes a self-limiting but sometimes severe diarrhea illness in immunocompetent humans.However，in those with compromised immune systems，such as AIDS patients，the disease can be debilitating，chronic and life threatening.Because of its ubiq-uitous distribution in the environment，its small size and its resistance to standard chlorination techniques，C.parvumcontamination of drinking water may pose a significant public health risk.To elucidate the pathogenic mechanisms ......