肝癌组织巨噬细胞移动抑制因子的表达及对肿瘤细胞增殖和血管形成的影响
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关泉林, 于晓辉, 霍永忠, 李 波
肝癌;巨噬细胞移动抑制因子;血管内皮生长因子;白细胞介素-8;微血管密度;免疫组织化学;实时定量聚合酶链反应,关泉林,于晓辉,霍永忠,李波,关泉林,通讯作者:,Expressionofmacrophagemigr
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关泉林, 兰州大学第一医院肿瘤外科 甘肃省兰州市 730000
于晓辉, 兰州军区兰州总医院消化科 甘肃省兰州市 730030
霍永忠, 广东省中医院普外科 广东省广州市 510120
李波, 四川大学华西医院普外科 四川省成都市 610041
关泉林, 2005年四川大学临床医学博士, 副教授, 主要从事消化道肿瘤的基础与临床研究.
通讯作者: 李波, 610041, 四川省成都市外南国学巷37号, 四川大学华西医院普外科. cdlibo@medmail.com
收稿日期: 2006-04-08 接受日期: 2006-04-29
Expression of macrophage migration inhibitory factor and its effect on cell proliferation and angiogenesis in human liver cancer
Quan-Lin Guan, Xiao-Hui Yu, Yong-Zhong Huo, Bo Li
Quan-Lin Guan,Department of Tumor Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
Xiao-Hui Yu,Department of Gastroenterology, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou 730030, Gansu Province, China
Yong-Zhong Huo,Department of General Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong Province, China
Bo Li,Department of General Surgery, West China Hospital, Sichuan University, Chendu 610041, Sichuan Province, China
Correspondence to:Bo Li, Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. cdlibo@medmail.com
Received:2006-04-08 Accepted:2006-04-29
Abstract
AIM: To determine the expression of macro-phage migration inhibitory factor (MIF) in hepatocellular carcinoma (HCC) and its effect on cell proliferation and angiogenesis.
METHODS: The protein expression of MIF, interleukin-8 (IL-8), and vascular endothelial factor (VEGF) as well as microvessel density (MVD) were examined by immunochemistry in HCC tissues (n = 40), cancer-adjacent liver tissues (n = 40) and normal liver tissues (n = 10). At the same time, real time quantitative polymerase chain reaction (RQ-PCR) was used to detect the mRNA levels of MIF, VEGF and IL-8. After human hepatocarcinama cell line Bel-7402 was treated with recombinant MIF (rMIF), cell proliferation was detected by MTT assay and the secretion levels and mRNA expression of VEGF and IL-8 were examined by enzyme linked immunosorbent assay (ELISA) and RQ-PCR, respectively ......
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