bFGF促进人大肠癌LoVo细胞增殖的信号转导机制
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周 颖, 王桂玲
碱性成纤维细胞生长因子;蛋白激酶B;CyclinA;大肠癌,周颖,王桂玲,周颖,通讯作者:,Signaltransductionmechanismofbasicfibroblastgrowthfactorinpromotingthegrow
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周颖, 王桂玲, 中国医科大学基础医学院细胞生物学教研室 辽宁省沈阳市 110001
周颖, 医学硕士, 助教, 主要从事肿瘤细胞信号转导方面的研究.
通讯作者: 王桂玲, 110001, 辽宁省沈阳市, 中国医科大学基础医学院细胞生物学教研室. wanggl2000@163.com
电话: 024-23256666-5347
收稿日期: 2006-09-28 接受日期: 2006-10-18
Signal transduction mechanism of basic fibroblast growth factor in promoting the growth of colorectal cancer LoVo cell line
Ying Zhou, Gui-Ling Wang
Ying Zhou, Gui-Ling Wang,Department of Cell Biology, Basic Medical College of China Medical University, Shenyang 11001, Liaoning Province, China
Correspondence to:Gui-Ling Wang, Department of Cell Biology, Basic Medical College of China Medical University, Shenyang 110001, Liaoning Province, China. wanggl2000@163.com
Received:2006-09-28 Accepted:2006-10-18
Abstract
AIM: To investigate the activity of protein kinase B (PKB) and the expression of Cyclin A in colorectal cancer LoVo cell line after treatment of basic fibroblast growth factor (bFGF), and to further analyze the signal transduction mechanism of bFGF in regulating the growth of LoVo cells via PI3-K/PKB pathway.
METHODS: Colorectal cancer cell line LoVo was cultured in vitro using routine method, and then treated with bFGF, LY294002 (inhibitor of PI3-K), and bFGF plus LY294002, respectively. MTT assay was used to determine the proliferaion of LoVo cells; PKB activity in LoVo cells was measured by (γ-32P)ATP incorporation assay; Cyclin A mRNA expression was examined by reverse transcription-polymerase chain reaction (RT-PCR); Cyclin A protein expression was detected by Western blot.
RESULTS: The activity of PKB was increased in LoVo cells after bFGF treatment at different time, but it was decreased by LY294002 pre-treatment [704.32 ± 12.35 pmol/(mg·min) vs 1352.79 ± 19.85 pmol/(mg·min),P < 0.05]. The expression of Cyclin A was significantly increased at both protein and mRNA levels in the cells treated by bFGF, but the up-regulation of Cyclin A was obviously inhibited by LY294002 pre-treatment ......
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