[摘要]目的 探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)的关系。方法 采用聚合酶链反应结合限制性片段长度多态分析方法，分析了100例健康人及110例冠心病病人的ApoH外显子3、8基因型。结果 冠心病组外显子3的GG基因型频率为81.8%，GA+AA基因型频率为18.2%，G等位基因频率为 88.0% ，A等位基因频率为12.0%，与对照组比较差异无显著性( χ 2 =2.147、1.454，P >0.05)。冠心病组外显子8的GG基因型频率为74.5%，GC基因型频率为25.5%，G等位基因频率为87.0%，C等位基因频率为13.0%，与对照组比较，冠心病组的GC基因型及C等位基因频率显著增高( χ2 =7.742、3.907，P <0.05)。结论 ApoH外显子3基因多态性与冠心病无相关性;ApoH外显子8 GC基因型及C等位基因与冠心病有关。

    [关键词] 载脂蛋白类;外显子;多态性，单核苷酸;冠状动脉疾病

    THE RELATIONSHIP BETWEEN APOLIPOPROTEIN H GENE POLYMORPHISM AND CORONARY HEART DISEASE

    WANG YONG-JUN， CUI GUO-FANG， ZHANG DIAN-WEN， et al

    (Department of Cardiology， Qingdao Hiser Medical Group， Qingdao 266033，China)

    [ABSTRACT]ObjectiveTo investigate the relationship between polymorphism of apolipoprotein H (ApoH) exon 3 and exon 8 with coronary heart disease (CHD). MethodsPolymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of ApoH exon 3 and exon 8 in 110 patients with CHD and 100 healthy adults. ResultsThe frequency of genotype GG of exon 3 in the CHD group was 81.8%; in GA+AA genotype ......