[摘要]目的 观察脑缺血再灌注对大鼠学习记忆能力及海马神经元谷氨酸(Glu)、NR1 、NR2B表达的影响，探讨脑缺血损伤与Glu兴奋性毒性的关系。方法 采用PULLINSIN-4VO改良法制备血管性痴呆动物模型，MORRIS水迷宫检测其空间学习记忆能力，苏木精-伊红染色显示海马组织结构变化，免疫组织化学SABC法显示海马神经元Glu及NR1 、NR2B的表达。结果 缺血15 min再灌注损伤48 h后，海马神经元大量坏死损伤，大鼠学习记忆能力明显下降，海马内Glu、NR1 和NR2B表达显著增加( F=22.063～1 368.839，q=3.453～5.342，P < 0.01 )。结论 缺血再灌注使大鼠学习记忆能力下降，其机制与海马神经元Glu、NR1 和NR2B的表达增加有关。

    [关键词] 再灌注损伤;学习;记忆;谷氨酸;海马;大鼠

    CHANGES OF LEARNING AND MEMORY ABILITIES AND EXPRESSION OF HIPPOCAMPUS Glu， NR1AND NR2BON CERE-BRAL ISCHEMIC REPERFUSION INJURY IN RATS

    ZHANG MIN， ZHOU YING-BIN， JIN YI

    (Department of Physiolo-gy， Qingdao University Medical College， Qingdao 266021， China)

    [ABSTRACT]ObjectiveTo observe the changes of learning and memory abilities and effects of expression of glutamate neu-trons (Glu)，NMDA receptor subunit NR1and NR2Bin hippocampus glutamate neutrons of rats with cerebral ischemic reperfusion (IR)， and to study the relationship between cerebral ischemia damage and glutamate excitability toxicity. MethodsThe im-proved PULLINSIN-4VO was applied to produce the models of vascular dementia， and MORRIS Maze test for the ability of space learning and memory ......