参麦注射液对心肌缺血再灌大鼠内皮细胞功能及结构的影响
参麦注射液,；心肌缺血；再灌注损伤；内皮细胞,,参麦注射液,；心肌缺血；再灌注损伤；内皮细胞,1材料和方法,2结果,3讨论,参考文献：
摘要：目的 研究参麦注射液(SM)对心肌缺血再灌注损伤(IR)大鼠内皮细胞的保护作用。方法 实验大鼠36只，随机分为3组：假手术对照组(Ⅰ)；心肌缺血再灌组(Ⅱ)；心肌缺血再灌+SM组(Ⅲ)。观察各组缺血30min再灌注3h后血循环内皮细胞数(CEC)、一氧化氮(NO)、内皮素(ET)的含量变化，同时电镜观察冠脉内皮细胞的超微结构。结果 ①与假手术组比缺血再灌组CEC增加(P<0.01)，NO下降(P<0.05)，ET含量增加(P<0.01)，内皮细胞的超微结构受损。②SM干预后CEC下降、NO上升明显(P<0.05)，ET显著减少(P<0.01)，内皮细胞的超微结构受到保护。结论 IR可诱发内皮功能紊乱，而SM能改善MIR损伤时的内皮形态及功能，对心肌具有保护作用。关键词：参麦注射液 ；心肌缺血；再灌注损伤；内皮细胞
Effects of Shenmai on Endothelial Cell in Myocardial IschemiaReperfusion in Rats
Li Jia
(Department of Pathophisiology，Xianning College,Hubei Xianning 437100,China)
ABSTRACT:Objective To study the protective effects of Shenmai (SM) on endothelial cell in myocardial ischemic reperfusion (MIR) in rats.Methods 36 rats were randomly divided into 3 groups(n=12 in each):sham operated group(Ⅰ), MIR group(Ⅱ) and SM group(Ⅲ). The number of circulation endothelial cells (CEC), plasma endothelin (ET),nitric oxide (NO) were measured after 30 minutes ischemia and 3 hours reperfusion , and the ultrastructure changes in arterial endothelial cells were observed under electron microscope. Results ①Comparing with group Ⅰ,the number of CEC increased (P<0.01),NO decreased(P<0.05)and ET increased (P<0.01)in groupⅡ and there were abnormal changes of the endothelial ultrastructure . ②Shenmai reversed the results of the above mentioned indices markedly.Conclusion Reperfusion results in dysfunction of endothelium (NO level decreasing and ET level increasing ), Shenmai reduces myocadial ischemioreperfusion injury by protecting endothelial structure and function. ......
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