摘要:目的 进一步探讨血清中的抗中性粒细胞胞浆抗体(ANCA)检测在肾脏疾病患者中的诊断意义。 方法 应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。 结果 在205例肾脏疾病患者血清中，ANCA阳性40例占19.5%，其中38例为pANCA阳性(38/205);2例为cANCA阳性(2/205)。 结论 不同ANCA荧光模型与疾病种类及预后密切相关;提高pANCA与ANA荧光模型的鉴别，有助于血管炎与其它自身免疫性疾病的诊断;同时应用IIF和ELISA方法检测ANCA，可以提高ANCA检测结果的可靠性。

    关键词:抗中性粒细胞胞浆抗体;间接免疫荧光;ELISA;肾脏疾病

    The significance of detection of ANCA from serum in diagnosis of nephropathy.

    FU Ke-ying，CHEN Ru，ZHONG Lu，et al.

    (Hainan Provincial People's Hospital，Haikou570311，Hainan，P.R.China)

    Abstract:Objective To further explore the significance of serum antineutrophil cytoplasmic antibodies(ANCA)in renal dis-eases. Methods ANCA fluorescent patterns and its target antigens in sera of205patients with renal diseases were determined by indirect immunofluorescence(IIF)and ELISA. Results The positive percentage of ANCA is19.5%(40/205) ......