增强型绿色荧光蛋白(EGFP)；转染；骨髓间充质干细胞(MS

EGFP基因转染骨髓间充质干细胞的实验研究

http://www.100md.com 《河北北方学院学报》 2006年第4期

增强型绿色荧光蛋白(EGFP)；转染；骨髓间充质干细胞(MSCs),,增强型绿色荧光蛋白(EGFP)；转染；骨髓间充质干细胞(MSCs),1材料和方法

     【摘要】目的: 探讨外源性EGFP基因转染修饰兔骨髓间充质干细胞(m esenchymal stem cells, MSCs)的可行性。方法: 用增强型荧光绿色蛋白(enhanced green fluorescent protein, EGFP)作为报告基因，以脂质体法转染骨髓间充质干细胞，观察转染结果、表达情况及对靶细胞活力的影响。结果: 经荧光显微镜下观察证实：成功地对骨髓间充质干细胞实现了基因转染。结论: 兔骨髓间充质干细胞能够在体外适宜的条件下进行长期培养; 骨髓间充质干细胞可直接作为基因靶细胞, 建立稳定表达EGFP 的骨髓间充质干细胞系具有可行性,为进一步做标记的MSCs细胞的移植研究奠定基础。

    【关键词】增强型绿色荧光蛋白(EGFP)；转染；骨髓间充质干细胞(MSCs)

    Study on Mesenchymal Stem Cells Transfected by EGFP

    ZHENG Liheng1, WEI Huiping2, XU Songmei1, et al

    1.Life Science College, Sichuan University, Chengdu, 610041, Sichuan, China;

    2.Hebei North University, Zhangjiakou, 075000, Hebei, China

    【ABSTRACT】 Objective: To study the feasibility of exogenous gene transfecting into bone marrow mesenchymal stem cells (MSCs). Methods: MSCs were cultured and treated with pEGFPC1 with the help of lipofectamine. The transgene results were confirmed by fluoroscopy and the cell viability after transfection was evaluated by light microscopy and electron microscopy. Results: Bone marrow MSCs were successfully transfected with pEGFPC1 gene. Conclusion: The MSCs originated from rat bone can be cultured in vitro under appropriate conditions, and they can be directly used as gene target cells. Moreover, it is feasible to construct the MSCs expressing EGFP stably, which can be further applied to label target cells in the transplantation study. ......

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