[摘要]目的 研究急性髓细胞白血病(AML)的端粒酶活性表达情况，探讨端粒酶活性检测对AML的临床意义。方法 选取不同亚型AML病人74例，以良性血液病和骨髓形态学正常的非血液病病人作对照，采用改良TRAP-银染法及亚利恩凝胶成像系统分析检测其骨髓标本内的端粒酶活性水平并随访。结果 良性血液病病人端粒酶阴性或低水平表达;AML初诊时端粒酶活性显著升高( F=36.76，q=14.65，P <0.01)，完全缓解后活性下降( q=10.75，P <0.01)，复发时端粒酶活性又升高( q=7.61，P <0.01)。同时端粒酶活性与预后相关，活性高者往往预后较差。结论 端粒酶活性与AML的发生、发展密切相关，可作为AML恶性克隆增殖的分子标志。

    [关键词] 端粒酶;白血病;聚合酶链反应

    CLINICAL SIGNIFICANCE OF TELOMERASE ACTIVITY IN PATIENTS WITH ACUTE MYELOCYTIC LEUKEMIA

    QIAO HONG， GUO XIAO-FANG， LU WEI

    (Department of Hematology， Qingdao University Medical College， Qingdao 266021， China)

    [ABSTRACT]ObjectiveTo study the expression of telomerase activity in patients with acute myelocytic leukemia (AML) and its clinical significance. MethodsAML patients ( n =74)， benign hematologic patients， and non-hematologic patients with normal bone marrow as controls， were included in this study. Modified TRAP-silver staining and Yalien analyzing software were used to analyze the corresponding data. ResultsExpression of telomerase of the controls was negative or low. For the AML pa-tients ......