[摘要]目的 采用多重聚合酶链反应(PCR)检测耐甲氧西林葡萄球菌(MRS)，并对我院葡萄球菌的耐药率进行调查。方法 对262株葡萄球菌(金黄色葡萄球菌86株，凝固酶阴性葡萄球菌176株)进行多重PCR扩增，并与琼脂稀释法作比较。对我院葡萄球菌的耐药率进行统计分析。结果 以mecA基因阳性判断MRS，灵敏度为100%，特异度为96.6%，阴性预测值为100%，阳性预测值为99.0%;以mecA基因和femA基因阳性判断耐甲氧西林金黄色葡萄球菌(MRSA)，灵敏度为100%，特异度为99.5%，阴性预测值为100%，阳性预测值为98.5%。我院葡萄球菌耐药率为78.6%，其中凝固酶阴性葡萄球菌耐药率为79.0%，金黄色葡萄球菌耐药率为78.0%。结论多重PCR技术检测mecA基因能快速、敏感、准确地检测MRS，与femA基因联合检测能有效检测MRSA。MRS已成为日益严重的葡萄球菌感染问题。

    [关键词] 葡萄球菌感染;甲氧西林抗药性;聚合酶链反应

    MULTIPLE PCR IN RAPID DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS

    MU XIAO-FENG， CHEN WEN， YAN ZHI-YONG， et al

    (Department of Clinical Laboratory， The Second Affiliated Hospital of Qingdao University Medical College， Qingdao 266042， China)

    [ABSTRACT]ObjectiveTo detect methicillin-resistant staphylococcus (MRS) by multiple PCR， and to investigate its drug resistance rate in our hospital. MethodsThe DNA fragments of 262 strains of staphylococcus (staphylococcus aureus， 86; co-agulase-negative staphylococcus， 176) were amplified by multiple PCR . The results were compared with Agar Dilution. The drug resistance rate was analyzed statistically. ResultsTaking mecA positive for MRS detection ......