人肝癌细胞系HepG2在遗传毒物检测中的应用及其进展
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姚晓峰, 仲来福
人肝癌细胞系;HepG2细胞系;遗传毒物;体外遗传毒性实验,姚晓峰,仲来福,通讯作者:,电话:, 传真:,收稿日期:,接受日期:,ApplicationofhumanhepatomacelllineHepG2anditspr
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姚晓峰, 仲来福, 大连医科大学毒理学研究室 辽宁省大连市 116027
通讯作者: 仲来福, 116027, 辽宁省大连市沙河口区中山路465号, 大连医科大学毒理学研究室. zhong@dlmedu.edu.cn
电话: 0411-84720583 传真: 0411-84720661
收稿日期: 2006-10-25 接受日期: 2006-11-02
Application of human hepatoma cell line HepG2 and its progress in the detection of genotoxicants
Xiao-Feng Yao, Lai-Fu Zhong
Xiao-Feng Yao, Lai-Fu Zhong, Department of Toxicology, Dalian Medical University, Dalian 116027, Liaoning Province, China
Correspondence to: Lai-Fu Zhong, Department of Toxicology, Dalian Medical University, 465 Zhongshan Road, Shahekou District, Dalian 116027, Liaoning Province, China. zhong@dlmedu.edu.cn
Received: 2006-10-25 Accepted: 2006-11-02
AbstractGenotoxicity test is widely used in the detection of various carcinogens and mutagens. HepG2 is derived from human hepatoblastoma, and it retains the activities of drug-metabolizing enzymes. It has been demonstrated that various carcinogens can be detected in genotoxicity test with HepG2 cells at several endpoints, whereas negative results have been obtained with non-carcinogens.
Key Words: Human hepatoma cell line; HepG2 cell line; Genotoxicant; In vitro genotoxicity test
Yao XF, Zhong LF. Application of human hepatoma cell line HepG2 and its progress in the detection of genotoxicants. Shijie Huaren Xiaohua Zazhi 2007;15(2):145-150
摘要外来化合物的体外遗传毒性实验常用于各种致癌物和致突变物的快速筛选. HepG2是一种分化好的人肝胚细胞瘤细胞系, 保留了较完整的代谢酶及其活性. 以HepG2细胞作为实验系统检测各种致癌及非致癌物, 在多个观察终点均获得相应的阳性及阴性结果.
关键词: 人肝癌细胞系; HepG2细胞系; 遗传毒物; 体外遗传毒性实验
姚晓峰, 仲来福. 人肝癌细胞系HepG2在遗传毒物检测中的应用及其进展. 世界华人消化杂志 2007;15(2):145-150
0 引言
一直以来, 遗传毒物的检测普遍受到人们的重视. 对于体外遗传毒性实验来说, 细胞的代谢系统将在很大程度上影响实验的结果. 而人肝癌细胞系HepG2保留了较完整的生物转化代谢Ⅰ相酶和Ⅱ相酶[1], 这意味着该细胞系既可作为外来化合物攻击遗传物质的靶细胞, 同时又是外来化合物的活化系统, 不需依赖传统外源性活化系统的加入. 自从1979年, Aden et al[2]从一个阿根廷男孩的原发性肝胚细胞瘤中分离出HepG2细胞, 科研人员多年来对其在遗传毒理学实验中的应用进行了深入的探索和广泛的研究 ......
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