一氧化氮合酶抑制剂对大肠癌裸鼠移植瘤的抗血管生成作用
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孙文洲, 于丽波, 董新舒
大肠癌;N-硝基精氨酸甲酯;肿瘤血管形成孙文洲,于丽波,董新舒.一氧化氮合酶抑制剂对大肠癌裸鼠移植瘤的抗血管生成作用. 世界华人消化杂志2007;15(2)114-117,孙文洲,于丽波,董新舒,孙文洲,通讯作者:,收稿日
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孙文洲, 于丽波, 董新舒, 哈尔滨医科大学附属肿瘤医院内科 黑龙江省哈尔滨市 150081
孙文洲, 副主任医师, 博士, 主要从事恶性肿瘤的临床与基础研究.黑龙江省青年科学技术专项资金项目, No. QC05C39
通讯作者: 孙文洲, 150081, 黑龙江省哈尔滨市, 哈尔滨医科大学附属肿瘤医院内科. ylbswz@yahoo.com.cn
收稿日期: 2006-10-10 接受日期: 2006-10-14
Anti-angiogenic effect of nitric oxide synthase inhibitor on colorectal cancer xenografts in nude mice
Wen-Zhou Sun, Li-Bo Yu, Xin-Shu Dong
Wen-Zhou Sun, Li-Bo Yu, Xin-Shu Dong, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Supported by the Special Fund for the Scientific and Technological Youth of Heilongjiang Provice, No. QC05C39
Correspondence to: Wen-Zhou Sun, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China. ylbswz@yahoo.com.cn
Received: 2006-10-10 Accepted: 2006-10-14
AbstractAIM: To investigate the anti-angiogenic effect of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase on the growth of colorectal cancer xenografts in nude mice.
METHODS: The xenografts derived from colorectal carcinoma cells LS174 were inoculated in nude mice, and then the mice were randomly divided into group A, B, C and D, intragastrically treated with normal saline (n = 6, 0.2 mL), L-NAME (n = 6, 1.5 mg/kg), 5-fluorouracil (5-FU, n = 6, 5 mg/kg), and L-NAME plus 5-FU (L-NAME 1.5 mg/kg, 5-FU 5 mg/kg), respectively, for 2 weeks (three times per week). Tumor volume was measured in nude mice bearing xenografts. The microvessel density (MVD) was determined by immunohistochemistry, and the protein level of vascular endothelial growth factor (VEGF) was detected by Western blot.
RESULTS: The tumor inhibition rates were significantly higher in group B, C, and D than that in group A (34.1%, 46.0%, 74.1% vs 0,P < 0.01). There was significant difference between group B and D as well as between group C and D (P < 0 ......
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