http://www.100md.com 高 玉, 纪 静, 朱 伟, 王 云, 罗 兵
高玉, 纪静, 王云, 罗兵, 青岛大学医学院微生物教研室 山东省青岛市 266021
朱伟, 济宁医学院微生物教研室 山东省日照市 276826国家自然科学基金资助项目, No. 30471623
通讯作者: 罗兵, 266021, 青岛市登州路38号, 青岛大学医学院微生物教研室. firstname.lastname@example.org
电话: 0532-83812423 传真: 0532-83812423
收稿日期: 2006-09-28 接受日期: 2006-11-08
Influence of Epstein-Barr virus latent membrane protein 2A on the proliferation of human gastric cancer cells
Yu Gao, Jing Ji, Wei Zhu, Yun Wang, Bing Luo
Yu Gao, Jing Ji, Yun Wang, Bing Luo, Department of Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong Province, China
Wei Zhu, Department of Microbiology, Jining Medical College, Rizhao 276800, Shandong Province, China
Supported by National Natural Science Foundation of China, No. 30471623
Correspondence to: Bing Luo, Department of Microbiology, Qingdao University Medical College, Number 38 of Dengzhou Road, Qingdao, 266021, Shandong Province, China. email@example.com
Received: 2006-09-28 Accepted: 2006-11-08
AbstractAIM:To construct recombinant adenovirus expression vector containing Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) and investigate the effects of LMP2A on the proliferation of human gastric cancer cell line.
METHODS: The target gene LMP2A was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the recombinant adenovirus vector carrying LMP2A gene was constructed with AdEasy system. The recombinant adenovirus vector pAd-2A was transfected into HEK293 cells with lipofectamine 2000 to package recombinant adenovirus, and then gastric cancer cells SGC with negative EBV were infected by recombinant adenovirus. The expression of LMP2A and its effect on the proliferation of SGC cells were analyzed by PT-PCR, MTT assay, fluorescence activated cell sorting (FACS) and confocal microscopy.
RESULTS:The recombinant adenovirus vector pAd-2A was successfully constructed and identified by PCR, restrictive enzyme analysis, and DNA sequencing ......