细胞增殖;_乳腺癌;(期刊论文)

辛基酚对MDAMB231乳腺癌细胞周期及周期蛋白表达的影响

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细胞增殖;,乳腺癌;,辛基酚；细胞周期蛋白D1;细胞周期蛋白质依赖激酶类,,细胞增殖;,乳腺癌;,辛基酚；细胞周期蛋白D1;细胞周期蛋白质依赖激酶类,辛基酚对MDAMB231乳腺癌细胞周期及

     Effects of octylphenol on cell cycle and cyclin expression of MDAMB231 breast cancer cells

    PENG JunHua, ZHANG Feng, ZHANG HuaXin, ZHANG QuanHua, ZHAO Yong, HE XiaoQian

    Department of Clinical Laboratory, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 730050, China

    【Abstract】 AIM: To observe the effects of octylphenol (OP) on cell cycle and cyclin expression of MDAMB231 breast cancer cells and to probe the molecular mechanisms of OP inhibiting MDAMB231 breast cancer cell proliferation. METHODS: The changes of cell proliferation, cell cycle, cyclinD1 mRNA, cyclinD3 mRNA, CDK2 mRNA, CDK4 mRNA and cyclinD1 protein expressions of MDAMB231 cells treated with OP were observed by using MTT test, flow cytometry, immunocytochemistry and RTPCR. RESULTS: When MDAMB231 breast cancer cells were treated with 4 or 8 μmol/L OP for 48 h, the cell inhibition rates were (17.13±4.1)%, (40.25±8.7)%, and the fluorescence values of cyclin D1 were 55.1±10.2, 41.4±11.2, significantly decreased as compared with control group(89.9±11.2, P<0.05). When MDAMB231 breast cancer cells were treated with 8 μmol/L OP for 24 and 72 h, the cell ratios in the G0/G1 phase were (67.0±17.6)% and (44.4±11.9)% ......

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