HBV相关性肝细胞癌及肝硬化患者外周血单个核细胞质谱分析
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钟白云, 范学工, 刘蔚东, 黄 燕, 邹明祥
肝细胞癌;乙型肝炎病毒;单个核细胞;肝硬化;蛋白质组;基质辅助激光解吸电离飞行时间质谱,钟白云,范学工,黄燕,邹明祥,刘蔚东,钟白云,通讯作者:,Massspectrometricanalysisofperipheralblood
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钟白云, 范学工, 黄燕, 邹明祥, 中南大学湘雅医院感染病科 湖南省长沙市 410008
刘蔚东, 中南大学湘雅医院卫生部肝胆肠外科研究中心 湖南省长沙市 410008
钟白云, 1998年中南大学湘雅医学院硕士, 副教授, 现在中南大学湘雅医院攻读博士, 从事肝癌的早期诊断及感染与免疫的研究.
通讯作者: 范学工, 410008, 湖南省长沙市湘雅路87号, 中南大学湘雅医院感染病科. xgfan@hotmail.com
电话: 0731-4327392
收稿日期: 2006-08-22 接受日期: 2006-09-25
Mass spectrometric analysis of peripheral blood mononuclear cells in patients with hepatitis B virus associated hepatocellular carcinoma or liver cirrhosis
Bai-Yun Zhong, Xue-Gong Fan, Wei-Dong Liu, Yan Huang, Ming-Xiang Zou
Bai-Yun Zhong, Xue-Gong Fan, Yan Huang, Ming-Xiang Zou,Department of Infectious Diseases, Xiangya Hospital of Central South University, Changsha 410008, Hu’nan Province, China
Wei-Dong Liu,National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Xiangya Hospital of Central South University, Changsha 410008, Hu’nan Province, China
Correspondence to:Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital of Central South University, 87 Xiangya Road, Changsha 410008, Hu’nan Province, China. xgfan@hotmail.com
Received: 2006-08-22 Accepted: 2006-09-25
Abstract
AIM: To establish two-dimensional electrophoresis (2-DE) maps of peripheral blood mononuclear cells (PBMC) in patients with hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC) or liver cirrhosis (LC), and identify the differentially expressed proteins.
METHODS: The total proteins from PBMC in patients with HBV associated HCC (n = 16) or LC (n = 12) were seperated by means of immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened using PDQuest analysis software, and then identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching.
RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC ......
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