急性胆道梗阻大鼠肝细胞的死亡方式
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徐 锋, 戴朝六, 贾昌俊, 许永庆, 彭松林, 顾 玺, 赵 阳
梗阻性黄疸;肝脏;胀亡;凋亡,徐锋,戴朝六,贾昌俊,许永庆,彭松林,顾玺,赵阳,徐锋,通讯作者:,Patternsoflivercelldeathinratswithacutebileductobstruction,FengXu,Chao-LiuDai
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徐锋, 戴朝六, 贾昌俊, 许永庆, 彭松林, 顾玺, 赵阳, 中国医科大学盛京医院肝胆外科 辽宁省沈阳市 110004
徐锋, 2006年中国医科大学博士研究生毕业, 讲师, 主要从事肝胆疾病基础与临床研究.
通讯作者: 戴朝六, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学盛京医院肝胆外科. daicl-sy@tom.com
电话: 024-83955232
收稿日期: 2006-10-17 接受日期: 2006-11-16
Patterns of liver cell death in rats with acute bile duct obstruction
Feng Xu, Chao-Liu Dai, Chang-Jun Jia, Yong-Qing Xu, Song-Lin Peng, Xi Gu, Yang Zhao
Feng Xu, Chao-Liu Dai, Chang-Jun Jia, Yong-Qing Xu, Song-Lin Peng, Xi Gu, Yang Zhao, Department of Hepatobiliary Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to:Professor Chao-Liu Dai, Department of Hepatobiliary Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. daicl-sy@tom.com
Received:2006-10-17 Accepted:2006-11-16
Abstract
AIM: To investigate the patterns of hepatic cell death in rats with acute bile duct obstruction.
METHODS: Male Wistar rats were divided randomly into bile duct ligation (BDL) group (n = 5) and control group (n = 5). The rats in BDL group underwent ligation and division of common bile duct to set up acute bile duct obstruction model according to Yoshidome’s maneuver, while the rats in the control group underwent dissociation of common bile duct only. All the animals were sacrificed for sampling on day 7. Flow cytometry was used to determine the percentages of hepatocyte apoptosis and oncosis. Hematoxylin and eosin (H&E) staining and electron microscopy were used to observe hepatocyte apoptosis, oncosis and histopathological changes. An auto analyzer was used to examine the levels of serum total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), glutamyl transpeptidase (γ-GT), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH).
RESULTS: The percentage of liver cell oncosis or necrosis was significantly higher in BDL group than that in the control group (oncosis: 25 ......
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