急性肝损伤大鼠肝脏血红素加氧酶-1/一氧化碳系统的变化及意义
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吴 煜, 李 琳, 温 韬, 李跃旗
肝损伤;血红素加氧酶-1;一氧化碳;D-氨基半乳糖;脂多糖,吴煜,李琳,李跃旗,温韬,吴煜,通讯作者:,Changesinexpressionofhemeoxygenase-1andproductionofcarbonmono
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吴煜, 李琳, 李跃旗, 解放军302医院门诊部 北京市 100039
温韬, 北京大学第三医院消化中心 北京市 100083
吴煜, 主治医师, 研究方向为肝病的发病机制及诊治.
通讯作者: 李跃旗, 100039, 北京市, 解放军302医院门诊部. springmaze@gmail.com
电话: 010-66933391
收稿日期: 2006-09-08 接受日期: 2006-11-16
Changes in expression of heme oxygenase-1 and production of carbon monoxide in rats with acute liver injury induced by D-galactosamine and lipopolysaccharide
Yu Wu, Lin Li, Tao Wen, Yue-Qi Li
Yu Wu, Lin Li, Yue-Qi Li,Department of infectious diseases, the 302th Hospital of Chinese PLA, Beijing 100039, China
Tao Wen,Research Center of Digestive Diseases, the Third Hospital of Peking University, Beijing 100083, China
Correspondence to:Yue-Qi Li, Department of Infectious Diseases, the 302th Hospital of Chinese PLA, Beijing 100039, China. springmaze@gmail.com
Received:2006-09-08 Accepted:2006-11-16
Abstract
AIM: To investigate the changes of heme oxygenase-1 (HO-1) expression and carbon monoxide (CO) production in acute liver injury induced by D-galactosamine (GalN) and Lipopolysaccharide (LPS), and explore the role of HO-1/CO system in the pathogenesis of liver injury.
METHODS: GalN (700 mg/kg body weight) and LPS (50 μg/kg body weight) were used to establish the models of acute liver injury in male rats by intraperitoneal injection. Liver injury was assessed biochemically and histologically. HO-1 activity in liver homogenate was examined at different time points following GalN/LPS treatment and the expression of HO-1 protein was determined by Western blot. The activity of super oxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissues were also examined at the same time.
RESULTS: Administration of GalN/LPS caused severe hepatic damage, characterized by significant elevation of serum alanine aminotransferase (ALT: 10872.5 ± 708.5 nkat/L vs 1043.5 ± 247 ......
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