5-Aza-CdR对胃癌细胞系生长及Apaf-1基因异常甲基化的影响
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王贺玲, 王学清, 李 岩, 孙 军
5-Aza-CdR;Apaf-1基因;胃癌;甲基化;细胞凋亡;细胞增殖,王贺玲,王学清,李岩,孙军,王贺玲,通讯作者:,Effectsof5-aza-2’-deoxycitydineonproliferationofhum
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王贺玲, 王学清, 李岩, 孙军, 中国医科大学第二附属医院消化内科 辽宁省沈阳市 110041
王贺玲, 博士研究生, 主要从事胃癌的临床研究.
通讯作者: 李岩, 110004, 辽宁省沈阳市, 中国医科大学第二附属医院消化内科. yanli0227@126.com
收稿日期: 2006-11-09 接受日期: 2006-11-28
Effects of 5-aza-2’-deoxycitydine on proliferation of human gastric cancer cell lines and abnormal methylation of Apaf-1 gene
He-Ling Wang, Xue-Qing Wang, Yan Li, Jun Sun
He-Ling Wang, Xue-Qing Wang, Yan Li, Jun Sun,Department of Gastroenterology, the Second Affiliated Hospital of China Medical University, Shengyang 110004, Liaoning Province, China
Correspondence to: Yan Li,Department of Gastroenterology, the Second Affiliated Hospital of China Medical University, Shengyang 110004, Liaoning Province, China. yanli0227@126.com
Received:2006-11-09 Accepted:2006-11-28
Abstract
AIM: To observe the effects of 5-aza-2’-deoxycitydine (5-Aza-CdR) on the proliferation of human gastric cancer cell lines SGC7901 and BGC823 as well as the methylation and epression of Apaf-1 gene.
METHODS: Human gastric cancer cell lines SGC7901 and BGC823 were cultured in RPMI1640 and then treated with different concentrations of 5-Aza-CdR (1 × 10-7, 5 × 10-7, 1 × 10-6 and 5 × 10-6 mol/L). The proliferation of the cells was detected by MTT assay and flow cytometry (FCM). The methylation of Apaf-1 gene in the two kinds of cell lines was detected by methylation-specific-polymerase chain reaction (MSP), and the expression of Apaf-1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Westen blot analysis.
RESULTS: 5-Aza-CdR displayed a growth inhibitory effect on SGC7901 and BGC823 cells in a dose- and time-dependent manner after exposure to 5-Aza-CdR at different concentrations (1 × 10-7, 5 × 10-7, 1 × 10-6, 5 × 10-6 mol/L) for 24, 48 and 72 hours. FCM analysis showed that the apoptosis rates in SGC7901 cells (2 ......
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