外源性转化生长因子β1对大鼠原代肝星状细胞活化的影响
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陈达凡, 李建英, 郑伟达, 陈治新, 王小众
转化生长因子β1;肝纤维化;肝星状细胞;a肌动蛋白,外源性转化生长因子β1对大鼠原代肝星状细胞活化的影响,陈达凡,李建英,郑伟达,陈治新,王小众,陈达凡,通讯作者:,Effectsofexogenoustransforminggrowthfactor-β1ontheactivationofp
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陈达凡, 李建英, 郑伟达, 陈治新, 王小众, 福建医科大学附属协和医院消化内科 福建省福州市 350001
陈达凡, 福建医科大学附属协和医院2004级硕士生, 主要从事消化系统疾病临床和研究.
福建省科技开发计划项目, No. 2005D094
通讯作者: 王小众, 350001, 福建省福州市新权路29号, 福建医科大学附属协和医院消化内科. drwangxz@pub6.fz.fj.cn
电话: 0591-83357896-8482
收稿日期: 2006-10-17 接受日期: 2006-11-28
Effects of exogenous transforming growth factor-β1 on the activation of primarily cultured rat hepatic stellate cells
Da-Fan Chen, Jian-Ying Li, Wei-Da Zheng, Zhi-Xin Chen, Xiao-Zhong Wang
Da-Fan Chen, Jian-Ying Li, Wei-Da Zheng, Zhi-Xin Chen, Xiao-Zhong Wang,Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China
Supported bythe Science and Technology Development Project of Fujian Province, No. 2005D094
Correspondence to:Xiao-Zhong Wang, Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, 29 Xinquan Road, Fuzhou 350001, Fujian Province, China. drwangxz@pub6.fz.fj.cn
Received:2006-10-17 Accepted: 2006-11-28
Abstract
AIM: To investigate the activation of primarily cultured hepatic stellate cells (HSCs) at different differentiated stages after treatment of exogenous transforming growth factor-β1 (TGF-β1) and the alterations of related factors during this process.
METHODS: HSCs were isolated from normal rats and primarily cultured in the uncoated plastics for 2, 3, 4, 5, 6, and 7 days. Then the cells were incubated with 5 mg/L exogenous TGF-β1 for 24 hours. The morphological features of the cells were observed under inverted microscope. Western blot was used to detect the changes of a-smooth muscle actin (a-SMA) after TGF-β1 treatment and the expression of transforming growth factor β1 recepter-Ⅱ (TβR-Ⅱ) during the activation of HSCs.
RESULTS: During the process of in vitro cultivation, HSCs at different differentiated stages showed different responses to exogenous TGF-β1 treatment ......
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