L02细胞在倒千里光碱处理大鼠肝中的增殖
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林 沪, 毛 青, 王宇明, 蒋业贵, 蒋 黎
肝细胞;嵌合肝;移植;大鼠;增殖;倒千里光碱林沪,毛青,王宇明,蒋业贵,蒋黎.L02细胞在倒千里光碱处理大鼠中的增殖. 世界华人消化杂志2007;15(4)346-352,林沪,毛青,王宇明,蒋业贵,蒋黎,林沪,硕士,主治医师,通讯作者
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林沪, 毛青, 王宇明, 蒋业贵, 蒋黎, 中国人民解放军第三军医大学西南医院感染病研究所 重庆市 400038
林沪, 硕士, 主治医师, 主要从事肝炎发病机制和病毒性肝炎动物模型的研究.国家自然科基金资助项目, No. 30271173
通讯作者: 毛青, 400038, 重庆市沙坪坝区高滩岩正街30号, 中国人民解放军第三军医大学西南医院感染病研究所.
qingmao@yahoo.com
电话: 023-65399064 传真: 023-68754475
收稿日期: 2006-11-20 接受日期: 2006-12-08
Repopulation of human L02 hepatocytes in tolerant rats treated with retrorsine
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Li Jiang
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Li Jiang, Institution of Infectious Diseases, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China
Supported by National Natural Science Foundation of China, No. 30271173
Correspondence to: Qing Mao, Institution of Infectious Diseases, Southwest Hospital, the Third Military Medical University, 30 Gaotanyan Center Street, Shapingba District, Chongqing 400038, China. qingmao@yahoo.com
Received: 2006-11-20 Accepted: 2006-12-08
AbstractAIM:To investigate whether human L02 hepatocytes can repopulate after transplantation into the normal and immunocompetent rats treated with retrorsine.
METHODS: Human L02 hepatocytes were injected into the peritoneal cavities of fetal Sprague Dawley rats to induce immune tolerance. The 3-week-old rats were injected with retrorsine ornormal saline. After DiI (1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethylindocarbocyanine perchlorate) staining, L02 hepatocytes were transplanted into the 7-week-old rats via the spleen. Immunofluorescent staining, reverse transcription-polymerase chain reaction (RT-PCR), S-P immunohistochemistry, and DiI staining were used to detect human albumin, human albumin mRNA and specific proliferating cell nuclear antigen (PCNA) in rat livers. The distribution of human L02 hepatocytes was observed under fluorescent microscope. The number density and area density of PCNA were obtained with the help of computer-assisted image analyzer ......
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