cnLocalization of NMDA and AMPAª²type glutamate receptors in forebrain ventricular membrane and subventricular zone of adult rats

    WEI Liª²Chun1, SHI Mei1, CAO Rong2, CHEN Liangª²Wei2

    1Center of Radiotherapy, Xijing Hospital, 2Institute of Neurosciences, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the distribution patterns of NMDAª²type and AMPAª²type glutamate receptor subunits in the forebrain ventricular membrane and subventricular zone of adult rats. METHODS: Immunohistochemical staining was applied to visualize the localization of 5 kinds of NMDA and AMPA receptor subunits in the forebrain sections of adult rats. RESULTS: The immunoreactivities for NMDAR1 (NR1), NR2C, AMPA receptor subunit 1(GluR1), GluR2/3, and GluR4 were detected in the ventricular membrane and subventricular zone. The immunoreactive products for NMDA or AMPA receptor subunits were homogeneously localized in the cytoplasma and cell membrane. Semiquantitative analysis indicated that NR1 and GluR4ª²immunoreactive cells were more and distributed more densely than NR2C, GluR1 or GluR2/3ª²immunopositive ones in ventricular membrane and subventricular zone. CONCLUSION: The localization of NMDA and AMPA receptor subunits in the ventricular membrane and subventricular zone suggests that NMDA and AMPA receptors may be involved in functional modulation of neurogenesis in above regions. ......