Correlation of p53 gene mutation and p16 gene deletion with hepatocellular carcinoma

    HU Juan, LIU Yuan

    Department of Clinical Laboratory ,General Hospital of Chengdu Military Area Command, Chengdu 610083, China, Graduate Brigade, Third Military Medical University, Chongqing 410038, China

    ¡¾Abstract¡¿AIM: To detect the probability of p53 gene mutation and p16 gene deletion in the plasma of the patients with hepatocellular carcinoma (HCC) and to explore its correlation with onset and development of HCC and its clinical significance. METHODS: PCR was used to amplify p53 gene exon 5ª²8 and the immunohistochemistry (SPª²p16) was employed to determine the deletion of p16 gene. The difference between the patients with or without liver metastasis was analysed in gene mutation and deletion. RESULTS: Of 33 cases of HLL 17(51.55%), showed the mutation of p53; the mutation rate in metastasis group(n=18) was higher than that in nonª²metastasis group(n=15). CONCLUSION: There is a correlation between HCC and p53 gene mutation and p16 gene deletion .The mutation rate may have the reference value in diagnosis and prognosis prediction of HCC. ......