Effect of matrine on expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats

    YANG Zhiª²Yun, YAO Shuª²Kun, YIN Fei

    2004 Doctor Postª²graduate Brigade, Hebei Medical University, Shijiazhuang 050017£¬China, Chinaª²Japan Friendship Hospital,Beijing 100029, China, Department of Gastroenterology, Fourth Affiliated Hospital, Hebei Medical University,Shijiazhuang 050011, China

    ¡¾Abstract¡¿ AIM: To study the expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats and the effect of matrine on them. METHODS: Fortyª²eight male SD rats were randomly separated into 4 groups according to body mass: model group(group A),lowª²dose matrine group( group B, 5 mg/100g), highª²dose matrine group (group C, 25 mg/100 g), control group (group D). The expression of Thyª²1 protein was detected by enzymehistochemisty, and the expressions of Notch1, Jagged1 mRNA were detected by semiª²quantitative RTª²PCR method. RESULTS: Compared with group D £¬the expressions of Thyª²1 protein, Notch1 mRNA, Jagged1 mRNA in group A were singnificantly increased (P<0.01). Compared with group A,the expressions of Thyª²1 protein, Notch1 mRNA,Jagged1 mRNA in group C were singnificantly increased(P<0.01). CONCLUSION: Matrine could regulate Notch signaling, decrease the expression of Thyª²1 protein,which might be one of the mechanisms of inhibiting the proliferation of hepatic oval cells and inducing rat hepatic oval cells to differentiate into mature hepatocytes. ......