灰树花；_多糖_β-葡聚糖；_酶_____灰树花；

灰树花子实体多糖中 β葡萄糖含量的酶法测定

http://www.100md.com 《时珍国医国药》 2006年第11期

灰树花；,多糖,β-葡聚糖；,酶,,灰树花；,多糖,β-葡聚糖；,酶,灰树花子实体多糖中β葡萄糖含量的酶法测定,1仪器与试剂,2方法与结果,3讨论,参考文献：

     摘要：目的探讨灰树花子实体多糖中 β葡聚糖含量的测定方法。方法多糖粗提物依次用高温 α淀粉酶、木瓜蛋白酶和 α1，4葡萄糖水解酶水解，通过测定其中葡萄糖的含量计算β葡聚糖的含量。结果确定了测定方法中几种酶的用量。在0~300 mg·L-1范围内线性关系良好，测得灰树花子实体多糖中 β葡聚糖含量为3.26%~3.51%，3组样品的平均回收率为90.5%。结论该方法操作简便，准确度高。

    关键词：灰树花；多糖 β-葡聚糖；酶

    Enzymatic Measurement of βglucans in Polysaccharides of Grifola frondosa

    CHEN Guoguang， MENG Lei， KONG Yi*,YANG Wan , Wu Wutong

    (College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology，Nanjing 210009, China；School of Life Science, China Pharmaceutical University, Nanjing, 210009, China)

    Abstract：ObjectiveAn enzymatic method for analysis of βglucan in polysaccharides of Grifola frondosa has been studied. MethodsAfter hydrolyzing by thermostable αamylose, papain and amyloglucosidase, the value of glucose were measured and the content of βglucan were computed .ResultsThe dosage of several enzymes were determined and the content of βglucan in the samples were in the range of 3.26%~3.51%. The average recovery was 90.5%. ConclusionThe enzymatic method is simple and with high accuracy. ......

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