柴胡；_高效液相色谱蒸发光散射检测法；_柴胡皂苷_

高效液相色谱蒸发光散射检测法测定柴胡药材中柴胡皂苷a及柴胡皂苷d的含量

http://www.100md.com 《时珍国医国药》 2006年第9期

柴胡；,高效液相色谱蒸发光散射检测法；,柴胡皂苷,,柴胡；,高效液相色谱蒸发光散射检测法；,柴胡皂苷,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的制定柴胡药材中柴胡皂苷a、d的含量测定方法。方法用高效液相色谱蒸发光散射检测法(HPLCELSD), Alltima C18色谱柱, 流动相甲醇水(80∶20),速度0.8 ml·min1；蒸发光散射检测器参数：漂移管温度40℃，气压3.5bar。结果柴胡皂苷a和柴胡皂苷d分别在1.908~19.08μg(r=0.999 6)和1.804~18.04 μg(r=0.999 2)范围内线性关系良好, 平均回收率分别为98.35%(RSD=1.26%)和97.64%(RSD=1.58%)。结论该方法简便、灵敏、可靠、准确、重现性好, 可作为柴胡药材的质量控制方法。

    关键词：柴胡；高效液相色谱蒸发光散射检测法；柴胡皂苷

    Determination of Saikosaponin a,d in Radix Bupleuri by HPLCELSD

    WANG Guangzhong， LI Qiuyi， ZHANG Lianping， LIU Yanwen*

    (Hubei College of Traditional Chinese Medicine,Wuhan 430061,China)

    Abstract：ObjectiveTo determine saikosaponin a,d in Radix Bupleuri by HPLCELSD. MethodsHPLCELSD was used in the quantitative analysis by using Alltima C18 chromatography column and methanolwater(80∶20) as a mobile phase. The flow rate of mobile phase was 0.8 ml·min1. The tube temperature of the detector was 40℃. The press of pure air was 3.5bar. ResultsThe linear ranges for saikosaponin a and d were 1.908~19.08μg(r=0.999 6)and 1.804~18.04μg(r=0.999 2), the average recoveries were 98.35% (RSD=1.26%)and 97.64%(RSD=1.58%), respectively. ConclusionThis method is easy, sensitive ,reliable, accurate ,reproducible and can be used as the quality control method of Radix Bupleuri. ......

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