黄芩苷；_黄芩水提物；_三波长紫外分光光度法_

水提物中黄芩苷含量的测定方法

http://www.100md.com 《时珍国医国药》 2006年第10期

黄芩苷；,黄芩水提物；,三波长紫外分光光度法,,黄芩苷；,黄芩水提物；,三波长紫外分光光度法,1仪器与材料,2方法,3结果,4讨论,参考文献：

     摘要：目的建立以水作溶剂的水系三波长紫外分光光度法，用于测定水提物中黄芩苷的含量，使之与水煎煮法提取黄芩苷工艺的溶剂体系相兼容，评价从黄芩中浸提黄芩苷的工艺并实现终产品的质量控制，以满足工厂大批量在线检测的需求。方法以水作溶剂的水系三波长紫外分光光度法，有别于前人以甲醇作溶剂的三波长紫外分光光度法，并进行了精密度实验，以验证方法的准确性。结果水溶黄芩苷标准溶液在0.5～12.0 μg/ml范围内，浓度与吸光度呈良好的线性关系，线性回归方程：△A＝0.032 77C0.009 08，相关系数r=0.997 08，三组样品测得的相对标准偏差RSD<2.1。结论以水作溶剂的水系三波长紫外分光光度法测定水提物中黄芩苷的含量是可行的，操作简便、快速、准确, 可以广泛应用于工业生产中,满足工业化需要,具有良好的应用前景。

    关键词：黄芩苷；黄芩水提物；三波长紫外分光光度法

    The Determination of Baicalin in the Water Extraction of Scutellaria baicalensis Georgi

    WANG Xiaoqin， ZHAO Ying， ZHI Dejuan, LI Hongyu

    (College of Life Sciences, Lanzhou University, Lanzhou 730000,China；Gansu Academy of Agricultural Sciences, Lanzhou 730000,China)

    Abstract：ObjectiveTo establish the water dissolved method of threewavelength UV spectrophotometry to determine baicalin content in water extraction of Scutellaria baicalensis Georgi.MethodsUsing the water dissolved method of threewavelength UV spectrophotometry, which differed from previous researches, then inspecting the accuracy by precision test. ResultsThe range of linearity with concentration and absorbance of standard baicalin dissolved in water was between 0.5～12.0 μg/ml, linearity relationship △A＝0.032 77C0.009 08, correlativity was 0.997 08, RSD < 2.1. ConclusionThe water dissolved method of threewavelength UV spectrophotometry to determine baicalin content in water extraction of Scutellaria Baicalensis Georgi is advisable, simple, rapid, accurate and has bright future on analysis application. ......

百拇医药网 http://www.100md.com/html/DirDu/2007/02/17/37/55/40.htm

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